一种测定巴西橡胶树树皮中过氧化氢含量的方法  

An Improved Technique for Determination of Hydrogen Peroxide in Bark of Rubber Tree(Hevea brasiliensis Muell. Arg.)

作　　者：杨署光[1] 陈月异[1] 李言[1] 张世鑫[1] 史敏晶[1] 田维敏[1] YANG Shuguang;CHEN Yueyi;LI Yan;ZHANG Shixin;SHI Minjing;TIAN Weimin(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences /Key Laboratory of Rubber Biology and Genetic Resources of Rubber Tree,Minstry of Agriculture and Rural Affairs /State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops,Danzhou,Hainan 571737,China)

机构地区：[1]中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737

出　　处：《热带作物学报》2018年第12期2383-2390,共8页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.20153136);中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022016003);国家天然橡胶产业技术体系分子育种岗位专项(No.CARS-34-GW1)

摘　　要：研究植物体内H_2O_2的含量变化有助于确认植物提高非生物胁迫耐性的生物技术策略。Ti(Ⅳ)-PAR-H_2O_2比色法灵敏度高、特异性强、稳定性好,广泛用于植物H_2O_2测定,但不同研究所用的反应条件不一致。在本研究中,系统优化了Ti(Ⅳ)-PAR-H_2O_2比色法的反应条件,并比较了丙酮提取法和TCA提取法对橡胶树萌条韧皮部H_2O_2的提取效果。结果表明,丙酮提取法稳定性差,TCA提取法稳定性好。本研究报道了一种简单、快速的测定橡胶树树皮H_2O_2的方法,为进一步研究橡胶树中H_2O_2的生理功能打下良好基础。Investigation of the H2O2 level induced by abiotic stress will be valuable for identifying biotechnological strategies to improve abiotic stress tolerance in crop plants.The Ti (Ⅳ)-PAR-H2O2 spectrophotometric method for determination of H2O2 was found to give good sensitivity,specificity and stability,and it has beenwidely used for estimation of H2O2 in plant extracts.However,various reaction conditions of this method were used by different authors.This led us to optimize the suitable condition of this method.For sample preparation,extraction with CCl4/CH3Cl3 (3 :1, V :V)and water in the proportion of 1 :3 :5 (V :V :V)after extracted with acetone were compared with the 5% the TCA extract method without activated charcoal in the bark of rubber trees.The 5%TCA method gives more stability than acetone method.In this paper,we described an easy and rapid protocol for determination of H2O2 in rubber tree bark using the Ti (Ⅳ)-PAR-H2O2 spectrophotometric method.Results could provide a basis for the investigation of the physiological function of H2O2 in rubber trees.

关 键 词：橡胶树 树皮 过氧化氢 测定 

分 类 号：S794.1[农业科学—林木遗传育种]