申克孢子丝菌TPS基因生物信息学分析  

Bioinformatics analysis of TPS gene from Sporothrix schenckii

作　　者：黄家敏[1] 李玉哲[2] 高文超[1] 吴晓雁 孙瑶[1] 黄丽林[3] 张静[1,4] 李美荣[1] 胡南[5] 旷翠娥[5] 刘佳庆 黄怀球[1] HUANG Jia-min;LI Yu-zhe2;GAO Wen-chao;WU Xiao-yan;SUN Yao;HUANG Li-lin;ZHANG Jing;LI Mei-rong;HU Nan;KUANG Cui-e;LIU Jia-qing;HUANG Huai-qiu(Department of Dermatology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Medical Laboratory,The Third Affiliated Hospital of Sun Yat-sen University-Lingnan Hospital,Guangzhou 510630,China;Department of Paediatrics,Guangdong Women and Children Hospital,Guangzhou 510010,China;Department of Dermatology,Sun Yat-sen Memorial Hospital of Sun Yat-sen University,Guangzhou 510120,China;Department of Dermatology,Shenzhen Baoan People's Hospital,Shenzhen 518101,China;Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第三医院皮肤科,广东广州510630 [2]中山大学附属第三医院岭南医院检验科,广东广州510630 [3]广东省妇幼保健院儿科,广东广州510010 [4]中山大学孙逸仙纪念医院皮肤科,广东广州510120 [5]深圳市宝安区人民医院皮肤科,广东深圳518101 [6]中山大学中山医学院,广东广州510080

出　　处：《皮肤性病诊疗学杂志》2018年第6期325-329,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金面上项目(编号:81371746);中山大学大学生创新训练计划项目(编号:201601072)

摘　　要：目的:分析和预测申克孢子丝菌海藻糖磷酸合成酶(TPS)基因及其编码蛋白的生物信息学特征及功能。方法:利用生物信息学软件和网站分析预测申克孢子丝菌TPS基因及其编码蛋白的结构和功能特征。结果:申克孢子丝菌TPS基因全长为2 864 bp,编码905个氨基酸。Target P和MotifScan预测该蛋白定位于胞浆中,具有糖基转移酶、海藻糖磷酸酶两大功能域及丰富的修饰位点,其中磷酸化位点尤为丰富,包含cAMP依赖性磷酸激酶、蛋白激酶C(PKC)和酪氨酸激酶(CK2)磷酸化位点。TPS蛋白是亲水性蛋白,无跨膜结构,未发现信号肽。结论:预测申克孢子丝菌TPS基因编码蛋白位于胞浆中,具有糖基转移酶、海藻糖磷酸酶两大功能域及丰富的修饰位点的结构功能特点,可行相应小分子抑制剂筛选以开发新型抗真菌药物。Objective:To analyze and predict the bioinformatics characteristics and functions of the trehalose phosphate synthase (TPS) gene and its encoding protein from Sporothrix schenckii. Methods:The bioinformatics software and professional online tools were utilized to analyze the characteristics of the TPS gene and its encoding protein from Sporothrix schenckii. Results:The TPS gene of Sporothrix schenckii is 2 864 bp in length and encodes 905 amino acids. TargetP and MotifScan predict that the protein is localized in the cytoplasm and has two functional domains of glycosyltransferase and trehalose phosphatase as well as abundant modification sites. The phosphorylation sites were particularly abundant, including cAMP-dependent phosphokinase, protein kinase C(PKC) and tyrosine kinase (CK2) phosphorylation sites. The TPS protein is a hydrophilic protein without transmembrane structure or signal peptide. Conclusions: It is predicted that the protein encoded by SsTPS gene is located in the cytoplasm which has two major functional domains of glycosyltransferase and trehalose phosphatase as well as rich modification sites. The outcome of the study will facilitate the screening of small molecule inhibitors to develop new antifungal drugs.

关 键 词：申克孢子丝菌 海藻糖磷酸合成酶 生物信息学 

分 类 号：R756[医药卫生—皮肤病学与性病学]