二氟甲基鸟氨酸对异丙肾上腺素诱导的大鼠心肌肥厚的影响  

Effect of α-difluoromethylornithine on isoproterenol induced cardiac hypertrophy in rats

作　　者：肖薇[1] 李波[1] 赵宇 王珺 贾伟伟 林岩[1] XIAO Wei;LI Bo;ZHAO Yu;WANG Jun;JIA Wei-wei;LIN Yan(School of Basic Medicine,Qiqihar Medical University,Qiqihar 161006, Heilongjiang Province,China)

机构地区：[1]齐齐哈尔医学院基础医学院,黑龙江齐齐哈尔161006

出　　处：《中国临床药理学杂志》2019年第1期39-41,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金应急管理基金资助项目(31751004);黑龙江省教育厅面上基金资助项目(2016-KYYWF-0846);黑龙江省科技厅面上基金资助项目(H2017080)

摘　　要：目的研究二氟甲基鸟氨酸(DFMO)对异丙肾上腺素诱导的大鼠心肌肥厚的影响。方法按照体重将大鼠随机分为3组:对照组、模型组和实验组,每组15只。实验组给予2%二氟甲基鸟氨酸,对照组与模型组皮下注射0. 9%Na Cl,均4周。以异丙肾上腺素5 mg·kg^(-1)连续皮下注射1周复制心肌肥大模型。计算心脏指数;用Msaaon染色评价纤维化程度;以实时荧光定量PCR测定心房钠尿肽(ANP)、鸟氨酸脱羧酶(ODC)和精脒/精胺乙酰转移酶(SSAT)mRNA水平。结果对照组、模型组、实验组的心脏指数(mg·g^(-1))分别为3. 49±0. 21,3. 76±0. 32和3. 55±0. 27;这3组的间质纤维化率分别为(6±2)%,(24±5)%和(14±2)%;这3组的ANP mRNA表达水平分别为0. 60±0. 08,1. 20±0. 44和0. 65±0. 13;这3组的ODC mRNA表达水平分别为0. 95±0. 19,1. 67±0. 21和1. 17±0. 09;这3组的SSAT mRNA表达水平分别为0. 95±0. 28,1. 57±0. 18和1. 85±0. 17。上述指标,模型组与对照组比较,差异均有统计学意义(均P <0. 05);实验组与模型组比较,差异均有统计学意义(均P <0. 05)。结论 DFMO对大鼠心肌肥厚有保护作用,其机制与降低多胺合成代谢和增强分解代谢有关。Objective To investigate the effect of α-difluoromethylornithine(DFMO)on cardiac hypertrophy induced by isoproterenol(ISO)in rats.Methods Wistar rats were randomly divided into 3 groups: control group,model group,and experimental group,15 rats in each group.The rats in experimental group were treated with 2% DFMO water for 4 weeks;the rats in control and model groups were treated with 0.9%NaCl,once a day for 4 weeks.Model rats was established by subcutaneously ISO 5 mg·kg^-1·d^-1,once daily for 1 week.The heart index was calculated.The interstitial fibrosis was evaluated by cardiac index and Masson staining.The mRNA expression of the atrial natriuretic peptide(ANP),ornithine decarboxylase(ODC),spermidine/spermine N1-acetyltransferase(SSAT)were determined by realtime polymerase chain reaction.Results The heart index(mg·g^-1)in control group,model group and experimental group were respectively 3.49±0.21,3.76±0.32,3.55±0.27;the ratio of collagen fibers in the 3 groups were respectively(6±2)%,(24±5)%,(14±2)%;the ANP mRNAin the 3 groups were respectively 0.60±0.08,1.20±0.44,0.65±0.13;the ODC mRNA in the 3 groups were respectively 0.95±0.19,1.67±0.21,1.17±0.09;the SSAT mRNA in the 3 groups were respectively 0.95±0.28,1.57±0.18,1.85±0.17.The difference of above factors between the model group and the control group was statistically significant(allP<0.05).The difference of above factors between the experimental group and the model group was statistically significant(allP<0.05).Conclusion DFMO had a protective effect on myocardial hypertrophy,and its mechanism was related to the reduction of anabolism and the enhancement of catabolism of polyamine.

关 键 词：心肌肥厚 多胺 鸟氨酸脱羧酶 二氟甲基鸟氨酸 

分 类 号：R97[医药卫生—药品]