薏苡的茎和叶石油醚萃取部位的分离组分抗肿瘤活性筛选研究  

Activity screening study on the antitumor effects of isolated components of petroleum ether extracts from stalks and leaves of Coix

作　　者：喻巧容 黄锁义[2,3] 李庭树 雷智冬 陈石梅 YU Qiao -rong;HUANG Suo -yi;LI Ting -shu;LEI Zhi -dong;CHEN Shi -mei(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China;Key Laboratory of Guangxi's College for The Study of Characteristic Medicine in Youjiang River Basin,Youjiang Medical University for Nationalities, Baise 533000,Guangxi Zhuang Autonomous Region,China;College of Pharmacy,Youjiang Medical University for Nationalities, Baise 533000,Guangxi Zhuang Autonomous Region,China;College of Basic Medicine,Youjiang Medical University for Nationalities, Baise 533000,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学药学院,南宁530001 [2]右江民族医学院广西高校右江流域特色民族药研究重点实验室,广西百色533000 [3]右江民族医学院药学院,广西百色533000 [4]右江民族医学院基础医学院,广西百色533000

出　　处：《中国临床药理学杂志》2019年第1期45-47,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81360684);国家中医药管理局"十二五"中医药重点学科中药化学建设基金资助项目(国中医药人教发[2012]32号);广西重点学科药物化学建设基金资助项目(桂教科研[2017]22号);广西自然科学基金资助项目(2011GXNSFA018046)

摘　　要：目的研究薏苡的茎和叶的石油醚萃取部位分离组分的抗肿瘤活性。方法薏苡茎叶的乙醇提取物经石油醚萃取后,以不同比例的石油醚-乙酸乙酯为流动相将石油醚相用硅胶柱色谱分离,所得洗脱液以乙酯比例10∶1石油醚-乙酸为展开剂用薄层色谱分析,合并相同极性段,各极性段经甲醇结晶处理共得到4个分离组分(样品1,2,3,4号),用CCK-8法检测这4个分离组分对3种肿瘤细胞株体外生长的抑制作用,并计算其半数抑制浓度(IC_(50)值)。结果从石油醚-乙酸乙酯比例20∶1的洗脱剂中得到了样品1号和4号,从石油醚-乙酸乙酯比例15∶1的洗脱剂中得到了样品2号和样品3号,4个样品均为干燥的混合物。4个样品对3种肿瘤细胞株的体外生长均有抑制作用,其中3号分离组分的抗肿瘤活性最好,3号样品对人宫颈癌(He La)、肝癌(Hep G2)、胃癌(SGC-7901)等细胞株的IC_(50)值分别为126. 1,145. 5,129. 2μg·m L^(-1)。结论薏苡的茎、叶乙醇提取物的石油醚萃取部位分离组分具有一定的抗肿瘤作用,其中3号样品抗肿瘤活性最好。Objective To explore the antitumor effects of isolated components of petroleum ether extracts from stalks and leaves of Coix.Methods The ethanol extracts from the stems and leaves of Coix were extracted by petroleum ether.The petroleum ether extracts were separated by silica gel column chromatography with different proportions of petroleum ether-ethyl acetate as mobile phase.The eluents were analyzed by thin layer chromatography(TLC)with petroleum ether-ethyl acetate ratio of 10∶ 1 as unfolding agent.The same polar segments were merged and four fractions(sample 1,2,3,4)were obtained by methanol crystallization.The in vitro growth inhibitory effects of the four fractions(sample 1,2,3,4)on 3 kinds of tumor cell lines was detected by Cell Counting Kit-8(CCK-8)assay.The half inhibitory concentration(IC50 value)was calculated.Results Sample 1 and sample 4 were obtained from the eluent with petroleum ether-ethyl acetate ratio of 20:1.Sample 2 and sample 3 were obtained from the eluent with petroleum ether-ethyl acetate ratio of 15:1.All the four samples were dry mixtures.The four samples can inhibit the growth of 3 kinds of tumor cell lines in vitro,and the No.3 isolate has the best anti-tumor activity.The IC50values of No.3 isolate on human cervical cancer(HeLa),liver cancer(HepG2)and gastric cancer(SGC-7901)cell lines were 126.1,145.5 and 129.2 μg·mL-1,respectively.Conclusion The isolated components of petroleum ether extracts from stalks and leaves of Coix have certain antitumor effect,the antitumor activity of No.3 isolate was the best.

关 键 词：薏苡茎叶 肿瘤细胞 抗肿瘤 活性筛选 

分 类 号：R28[医药卫生—中药学]