银杏叶提取物对大鼠臂丛根性撕脱再植后神经元轴突再生及再髓鞘化的影响  

Effects of Ginkgo biloba extract on neuronal axonal regeneration and remyelination in rats after replantation brachial plexus root avulsion

作　　者：韦勇 邓丹琼[2] 吴盛龙 林烈宝 黄飞飞 朱兵[1] 陈磊 陈秀丹 冯清伟 WEI Yong;DENG Dan -qiong;WU Sheng -long;LIN Lie -bao;HUANG Fei -fei;ZHU Bing;CHEN Lei;CHEN Xiu-dan;FENG Qing -wei(Department of Radiology,Hainan Maternal and Child Health Care Hospital,Haikou 570206,China;Department of Radiology,First Affiliated Hospital of Hainan Medical University, Haikou 570102,China)

机构地区：[1]海南省妇幼保健院放射科,海口570206 [2]海南医学院第一附属医院放射科,海口570102

出　　处：《中国临床药理学杂志》2019年第1期52-55,共4页The Chinese Journal of Clinical Pharmacology

基　　金：海南省自然科学基金资助项目(20168328; 20158308)

摘　　要：目的探讨银杏叶提取物对大鼠臂丛根性撕脱再植后神经元轴突再生及再髓鞘化的影响。方法选取健康雌性SD大鼠72只,其中54只用手术建立C5-C7臂丛神经撕脱、C6神经根再植模型,将造模成功大鼠随机分为模型组、对照组和实验组,每组18只;假手术组18只接受假手术。假手术组及模型组均给予0. 9%Na Cl 2 m L,qd,腹腔注射;对照组给予8. 5 mg·m L^(-1)氯化锂溶液2m L,qd,腹腔注射;实验组给予10. 0 mg·m L^(-1)银杏叶提取物溶液2 m L,qd,腹腔注射。各组均连续干预12周。对大鼠运动及感觉神经功能、再生运动神经元数量、肌皮神经再生神经轴突的数量和再髓鞘化程度,以及肌皮神经Slit1蛋白、Slit2蛋白表达水平进行检测。结果治疗后,实验组、对照组、模型组和假手术组的Terzis梳洗试验评分分别为(4. 21±0. 59),(3. 73±0. 51),(0. 22±0. 03)和(4. 83±0. 67)分,痛阈压力差分别为(1. 45±0. 16),(2. 26±0. 31),(4. 53±0. 62)和(0. 37±0. 05) N,停留时间差分别为(4. 02±0. 37),(6. 47±0. 75),(10. 85±1. 28)和(1. 53±0. 21) s,再生运动神经元数量分别为(414. 25±43. 63),(365. 54±35. 48),(175. 47±21. 17)和(82. 17±10. 23)个,肌皮神经轴突总数分别为(16. 64±2. 18),(13. 43±1. 53),(8. 65±1. 12)和(1. 62±0. 19)×10~3·mm^(-2),有髓轴突数量分别为(9. 27±1. 28),(6. 84±0. 73),(4. 37±0. 56)和(0. 83±0. 11)×103·mm^(-2),再生轴突髓鞘厚度分别为(0. 77±0. 11),(0. 65±0. 08),(0. 46±0. 06)和(0. 89±0. 12)μm,肌皮神经Slit1蛋白IOD值分别为(130. 59±14. 28)×10~2,(116. 84±12. 14)×10~2,(99. 75±11. 14)×10~2和(80. 32±10. 12)×10~2,Slit2蛋白IOD值分别为(224. 86±30. 45)×10~2,(165. 86±22. 85)×10~2,(127. 54±15. 45)×10~2和(104. 83±13. 24)×10~2,实验组的上述指标与假手术组、模型组、对照组比较,差异均有统计学意义(均P <0. 05)。结论银杏叶提取物能促进大鼠臂丛根性撕脱再植后神经元轴突再生及再髓鞘化,加速运动和感觉Objective To analysis the effect of ginkgo biloba extract on neuronal axonal regeneration and remyelination in rats after replantation brachial plexus root avulsion.Methods Seventy-two healthy female SD rats were selected,54 of them were successfully established C5-C7 brachial plexus avulsion and C6 nerve root replantation model C5-C7 brachial plexus avulsion and C6 nerve root replantation model by surgery,the model rats were randomly divided into model group,control groupand test group,each with 18 rats,and 18 rats in sham-operation group underwent sham surgery.The sham-operation group and model group were given physiological saline 2 mL by intraperitoneal-injection,qd;the control group was given 8.5mg·mL^-1 lithium chloride solution 2 mL by intraperitoneal-injection,qd;the test group was given1 0.0 mg·mL^-1 ginkgo biloba extract solution 2 mL by intraperitoneal-injection,qd.Each group received continuous intervention for 12 weeks.Motor and sensory neurological function,the number of regenerated motor neurons,the number of regeneration axons and the degree of re myelination in musculocutaneous nerve,the expression levels of Slit1 protein and Slit2 protein in musculocutaneous nerve were detected.Results After treatment,the main indexes of test,control,model and sham-operation groups were compared: the Terzis grooming test scores were(4.21±0.59),(3.73±0.51),(0.22±0.03)and(4.83±0.67)scores, the pain threshold pressure difference were(1.45±0.16),(2.26±0.31),(4.53±0.62)and(0.37±0.05)N,the residence time difference were(4.02±0.37),(6.47±0.75),(10.85±1.28)and(1.53±0.21)s,the number of regenerated motor neurons were(414.25±43.63),(365.54±35.48),(175.47±21.17)and(82.17±10.23)neurons,the total number of nerve axons were(16.64±2.18),(13.43±1.53),(8.65±1.12)and(1.62±0.19)×103·mm^-2,the number of myelinated axons were(9.27±1.28),(6.84±0.73),(4.37±0.56)and(0.83±0.11)×103·mm^-2,the myelin sheath thickness of regenerated axon were(0.77±0.11),(0.65±0.08),(0.46±0.06)and(0.89±0.12)μm,the IOD valu

关 键 词：银杏叶提取物 臂丛神经根性撕脱再植 神经元轴突再生 再髓鞘化 

分 类 号：R28[医药卫生—中药学]