Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3  

作　　者：MA Dong-fang HOU Lu SUN Cai ZHANG Xing YIN Jun-liang GUO Qing-yun ZHU Yong-xing 

机构地区：[1]Qinghai Academy of Agriculture and Forestry Sciences,Qinghai University/Key Laboratory of Agricultural Integrated Pest Management,Qinghai Province/State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University [2]Hubei Collaborative Innocvtion Center for Grain Industry/College of Agriculture,Yangtze University

出　　处：《Journal of Integrative Agriculture》2019年第1期108-114,共7页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China (31660513,31501620 and 31701911);the Provincial Natural Science Foundation of Qinghai,China (2017-ZJ-793)

摘　　要：Several new stripe rust pathogen races emerged in the wheat growing regions of China in recent years.These races were virulent to most of the designated wheat seedling resistance genes.Thus,it is necessary and worthwhile to identify new valuable resistant materials for the sake of diversifying resistant sources,pyramiding different resistance genes and achieving durable resistance.Here,we identified the resistance gene,temporarily designated as YrH9017,in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3.A total of 146 F2 plants and their derived F2:3 families in a cross of Mingxian 169 and H9017-14-16-5-3 were used to evaluate seedling stripe rust response and as a mapping population.Finally,we constructed a genetic map including eight simple sequence repeat(SSR) markers and expressed sequence tag(EST) markers.YrH9017 was located on the long arm of chromosome 2A and closely linked with two EST-sequence tagged site(EST-STS) markers BG604577 and BE471201 at 1.3 and 1.8 cM distance,respectively.The two closest markers could be used for marker-assisted selection of YrH9017 in breeding.Several new stripe rust pathogen races emerged in the wheat growing regions of China in recent years.These races were virulent to most of the designated wheat seedling resistance genes.Thus,it is necessary and worthwhile to identify new valuable resistant materials for the sake of diversifying resistant sources,pyramiding different resistance genes and achieving durable resistance.Here,we identified the resistance gene,temporarily designated as YrH9017,in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3.A total of 146 F2 plants and their derived F2:3 families in a cross of Mingxian 169 and H9017-14-16-5-3 were used to evaluate seedling stripe rust response and as a mapping population.Finally,we constructed a genetic map including eight simple sequence repeat(SSR) markers and expressed sequence tag(EST) markers.YrH9017 was located on the long arm of chromosome 2A and closely linked with two EST-sequence tagged site(EST-STS) markers BG604577 and BE471201 at 1.3 and 1.8 cM distance,respectively.The two closest markers could be used for marker-assisted selection of YrH9017 in breeding.

关 键 词：PUCCINIA STRIIFORMIS Psathyrostachys huashanica STRIPE RUST MOLECULAR mapping resistance gene 

分 类 号：S[农业科学]