RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析  

Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight,Serum Lipid and Acute Toxicity

作　　者：邱敏懿 晋小雁 张彩娟 张驰[1] 王敏[1] 卢彦冰 赵保胜[1] 张元[2] 王学勇[1] QIU Min-yi;JIN Xiao-yan;ZHANG Cai-juan;ZHANG Chi;WANG Min;LU Yan-bing;ZHAO Bao-sheng;ZHANG Yuan;WANG Xue-yong(School of Chinese Materia Medica,Beijing University of Chinese Beijing Research Institute of Chinese Medicine,Medicine,Belting 100029,China;College of Biochemical Engineering,Beijing Union University,Beijing 100023,China)

机构地区：[1]北京中医药大学中药学院,北京中医药研究院,北京100029 [2]北京联合大学生物化学工程学院,北京100023

出　　处：《中国实验方剂学杂志》2019年第1期149-156,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中央本级重大增减支项目(2060302);国家自然科学基金项目(81773841)

摘　　要：目的:评价芭蕉芋RS3型抗性淀粉对肥胖型高脂血症小鼠的减肥降脂作用,并进行安全性评价。方法:KKAy小鼠高脂饲料喂养20周,建立肥胖型高脂血症模型,随机分为模型组,辛伐他汀组(4mg·kg^-1),RS3抗性淀粉高、低剂量组(2,1g·kg^-1),并以维持饲料喂养的小鼠为正常组。给药组分别灌胃相应药物,正常组及模型组给予等量去离子水。8周后,处死小鼠,检测血清中总胆固醇(TC),甘油三酯(TG),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)及天门冬氨酸氨基转移酶(AST),丙氨酸氨基转氨酶(ALT)的含量,精密称量脂肪质量,计算脂/体比、体脂率及Lee's指数,伊红-苏木素(HE)染色观察肝脏及脂肪组织的病理学变化;采用限量法评价RS3抗性淀粉的安全性,连续观察14d,记录小鼠毒副反应情况。结果:RS3抗性淀粉高剂量能显著降低小鼠体质量、脂肪质量、体脂率、脂/体比、Lee's指数,以及血清中TC,TG,LDL-C,AST,ALT水平(P<0.05)。组织形态学检测显示,给予RS3抗性淀粉可以明显改善肝脏组织的脂肪病变情况,保肝作用明显,可以抑制脂肪细胞膨大,降低小鼠脂肪累积,以高剂量为优;急毒实验小鼠按36g·kg^-1剂量给药后,动物未出现中毒反应及死亡。结论:RS3型芭蕉芋抗性淀粉具有良好的减肥降脂作用,且以高剂量组为佳,最大给药量证明无毒副作用,临床常用剂量安全可靠。Objective: To evaluate effect of Canna edulis type 3 resistant starch(RS3) on weight loss and lipid reduction in obese hyperlipidemia mice and acute toxicity in mice. Method: KKAy mice were fed with high-fat diet for 20 weeks to establish a hyperlipidemia model and then randomly divided into model group,positive group(4 mg·kg^-1),high-dose resistant starch group and low-dose resistant starch group(2,1 g·kg^-1). Mice in normal group were fed with standard diet. The medication groups received corresponding drugs by gavage. Normal group and high-fat model group were given equal volume of deionized water. After 8 weeks,mice were put to death. The levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C), aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in serum of mice were measured,and weigh fat mass,fat/body ratio,body fat rate and Lee’s index were calculated accurately. The pathological changes of liver and adipose tissue were observed byhematoxylin-eosin(HE). The acute toxicity of RS3 to mice was evaluated by limit test. The mice were continuously observed for 14 days,and the toxicity of mice was recorded. Result: The indicators of high-dose RS3 group were significantly reduced,such as body weight,fat mass,body fat rate,fat/body ratio,Lee’s index,and serum TC,TG,LDLC,AST,ALT levels(P < 0. 05). Histomorphometric examination showed that the administration of RS3 starch could significantly improve the fatty lesions of liver tissue,and the liver-protecting effect was obvious,which could still inhibit the expansion of fat cells and reduce the accumulation of fat in mice. Among them,the high-dose group wsa better;After the maximum dose of 36 g·kg^-1 was administered,no toxic reaction and death occurred in the animals. Conclusion: RS3-type Canna Edulis Resistant Starch has a good effect in reducing body weight and serum lipid,with a better effect in the high-dose group and no toxicity. And the commonly used clinical dos

关 键 词：RS3型芭蕉芋抗性淀粉 肥胖症 高脂血症 降血脂作用 急性毒性 

分 类 号：R28[医药卫生—中药学] R285[医药卫生—中医学]