麻黄性味拆分组分对上焦水饮内停大鼠模型的影响  

Effect of Nature and Flavor Subdivision of Ephedrae Herba on Rats Model of Harmful Fluid Retention in Upper Jiao

作　　者：杨胜利[1] 孙亚萍[1] 司彦坡 韩杜菀 郑晓珂[1,2] 冯卫生 YANC Sheng-li;SUN Ya-ping;SI Yan-po;HAN Du-wan;ZHENG Xiao-ke;FENG Wei-sheng(Henan University of Traditional Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450046

出　　处：《中国实验方剂学杂志》2019年第3期1-7,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB531802)

摘　　要：目的:研究麻黄发挥作用的药效物质基础,并初步探讨其作用机制,进一步丰富麻黄的药性理论。方法:采用复合因素建立上焦水饮内停大鼠模型;将造模大鼠随机分为模型组,卡托普利组(4. 38 mg·kg^-1),麻黄水煎液组(468 mg·kg^-1),多糖组(265. 36 mg·kg^-1),挥发油组(2. 34 mg·kg^-1),生物碱组(40. 71 mg·kg^-1)和酚酸组(210. 60 mg·kg^-1),同时设立正常组,各组大鼠分别给予相应的药物(10 m L·kg^-1),正常组和模型组给予同等体积生理盐水,灌胃给药4周,采用大鼠代谢笼法收集24 h尿量,光镜下观察大鼠心和肺组织形态变化,并检测大鼠的心脏指数,肺脏指数,左室射血分数(LVEF),左室短轴缩短率(LVFS),肺通透指数(LPI),肺干湿比(W/D),肌酸激酶同工酶(CK-MB),血管紧张素Ⅱ(AngⅡ),醛固酮(ALD),心脏水通道蛋白-1(AQP1),肺脏AQP1,水通道蛋白-3(AQP3)和肾脏AQP1,AQP2,白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的变化。结果:与正常组比较,模型组大鼠心、肺组织受损较严重,模型大鼠24 h尿量,LVEF,LVFS均显著降低(P<0. 01);心脏指数,肺脏指数,CK-MB,LPI,AngⅡ,W/D,ALD,IL-6和TNF-α水平均明显升高(P<0. 05,P<0. 01),上焦水饮内停大鼠模型建立成功;与模型组比较,生物碱组能显著升高大鼠24 h尿量,LVEF,LVFS(P<0. 05,P<0. 01),显著降低心脏指数,肺脏指数,CK-MB,LPI,AngⅡ,W/D,ALD,IL-6和TNF-α水平(P<0. 05,P<0. 01),且对上焦水饮内停大鼠心、肺病理状况有明显改善作用。结论:麻黄生物碱组分"辛""温",是其发挥作用的药效物质基础,其作用可能与肾素-血管紧张素-醛固酮系统(RAAS)的抑制及其抗炎有关。Objective: To study the effective substance foundation of Ephedrae Herba and explore its mechanism,in order to further enrich the theory of drug resistance of Ephedrae Herba. Method: In this experiment,a compound model was used to establish rat model of Harmful Fluid Retention in upper Jiao. The Rats were randomly divided into model group,captopril group( 4. 38 mg·kg^-1),Ephedrae Herba decoction group( 468 mg·kg^-1),polysaccharide group( 265. 36 mg·kg^-1),volatile oil group( 2. 34 mg·kg^-1),alkaloid group( 40. 71 mg·kg^-1) and phenolic acid group( 210. 60 mg·kg^-1),and normal group( 10 m L·kg^-1). The normal group and the model group were given the same volume of normal saline for four weeks. The 24 h urine volume of rats was collected by metabolic cage method. The changes of heart and lung tissue morphology were observed under light microscope. The heart index,lung index,left ventricular ejection fraction( LVEF),left ventricular short axis shortening rate( LVFS) and pulmonary permeability index,number( LPI),lung dry-wet ratio( W/D),creatine kinase isoenzyme( CK-MB),angiotensin Ⅱ( Ang Ⅱ),aldosterone( ALD),cardiac aquaporin 1( AQP1),lung AQP1,aquaporin-3( AQP3) and kidney AQP1,aquaporin-2( AQP2),interleukin-6( IL-6) and tumor necrosis factor-α( TNF-α) change were detected. Result: Compared with the normal group,heart and lungs of the model group were significantly damaged. The amount of 24 h urine,LVEF,LVFS of model rats were significantly reduced( P< 0. 01);whereas heart index,lung index,CK-MB,LPI,Ang II,W/D,ALD,IL-6 and TNF-α were significantly increased( P< 0. 05,P< 0. 01),and the model of Harmful Fluid Retention in the Upper Jiaowas established successfully. The amount of 24 h urine,LVEF,LVFS,IL-6 and TNF-α were significantly increased( P< 0. 05,P< 0. 01) in the alkaloid group,and the heart index,the lung index,CK-MB,LPI,Ang Ⅱ,W/D,ALD,IL-6 and TNF-α were significantly reduced( P< 0. 05,P< 0. 01). And the Alkaloid group can alleviate the heart and lung pathology in the Rats Model of Harmful Fl

关 键 词：麻黄性味拆分组分 上焦水饮内停 肾素-血管紧张素-醛固酮系统(RAAS) 

分 类 号：R20[医药卫生—中医学] R22