风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响  

Effect of Fengshi Qutong Capsule on Collagen-induced Arthritis of Rats

作　　者：王靖霞 刘春芳[2] 何莲花[2] 孙丛丛[2] 李逸群 孟昊业[3] 彭江[3] 荆宇 苗艳东 林娜[1,2] WANG Jing-xia;LIU Chun-fang;HE Lian-hua;SUN Cong-cong;LI Yi-qun;MENG Hao-ye;PENG Jiang;JING Yu;MIAO Yan-dong;LIN Na(Chengde Medical University,Chengde 067000,China;Institute of Chinese Materia Medica,China Academy of Chinese Medicine Sciences,Beijing 100700,China;Institute of Orthopaedics,Chinese PLA General Hospital,Beijing 100853,China;Tonghua Golden-Horse Group,Beijing 100028,China)

机构地区：[1]承德医学院,河北承德067000 [2]中国中医科学院中药研究所,北京100700 [3]中国人民解放军总医院骨科研究所,北京100853 [4]通化金马药业集团股份有限公司,北京100028

出　　处：《中国实验方剂学杂志》2019年第2期89-95,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院中药研究所技术研发项目(20171011)

摘　　要：目的:观察风湿祛痛胶囊对大鼠胶原诱导性关节炎(CIA)的干预作用。方法:SD大鼠随机分为正常组、模型组、风湿祛痛胶囊低、中、高剂量组(0. 25,0. 5,1 g·kg^-1·d^-1),对照药甲氨蝶呤组(0. 2 mg·kg^-1·d^-1),除正常组外,其他各组以Ⅱ型胶原和不完全弗氏佐剂免疫大鼠建立CIA模型,首次免疫后第1天给药组开始灌胃给药,每天1次,共给药19 d;首次免疫第8天开始观察大鼠关节红肿和畸形症状、评价关节炎临床积分和发病率,第19天取材后,炎症关节进行微计算机断层扫描技术检测和骨计量学分析,同时进行炎症关节组织病理学检查并从滑膜炎症、血管翳、软骨侵蚀和骨破坏等方面进行半定量评分,酶联免疫吸附测定法检测血清白细胞介素-1β(IL-1β),肿瘤坏死因子-α(TNF-α),血管内皮生长因子(VEGF),基质金属蛋白酶-3(MMP-3)和核转录因子-κB受体活化因子配基(RANKL)含量。结果:风湿祛痛胶囊能剂量依赖地改善CIA大鼠炎症关节红、肿和畸形症状;与正常组比较,模型组大鼠炎症关节红、肿和畸形明显临床积分和发病率、关节滑膜炎症、血管翳、软骨侵蚀和骨破坏的病理评分显著升高(P <0. 01),炎症关节骨密度(BMD),骨体积分数(BV/TV),骨小梁厚度(Tb. Th),骨小梁数量(Tb. N)显著降低(P <0. 01),骨表面积体积比(BS/BV)和骨小梁分离度(Tb. Sp)显著升高(P <0. 01),血清IL-1β,TNF-α,VEGF,MMP3和RANKL的含量显著升高(P <0. 01);与模型组相比,各风湿祛痛胶囊组红肿、畸形症状明显改善,临床积分和发病率、关节滑膜炎症、血管翳、软骨侵蚀和骨破坏的病理评分均明显降低(P <0. 05,P <0. 01),BMD,BV/TV,Tb.Th,Tb. N明显升高(P <0. 05,P <0. 01),BS/BV和Tb. Sp明显降低(P <0. 05,P <0. 01),血清IL-1β,TNF-α,VEGF,MMP-3和RANKL的含量明显降低(P <0. 05,P <0. 01),且甲氨蝶呤组与高剂量组的作用相近。结论:风湿祛痛胶囊能有效缓解CIA的临床症状和病情,降�Objective: To observe the intervention effect of Fengshi Qutong capsule on collagen-induced arthritis( CIA) in rats. Method: SD rats were randomly divided into normal group,model group,low,medium and high-dose Fengshi Qutong capsule groups( 0. 25, 0. 5, 1 g·kg^-1·d^-1), and methotrexate group( 0. 2 mg·kg^-1·d^-1). Except for normal group,the other groups were immunized with type Ⅱ collagen and incomplete Freund's adjuvant to establish a CIA model. On the 1 stday after the first immunization, the administration group was given intragastric administration,once a day,for 19 days;on the 8 thday after the first immunization,the symptoms of joint swelling and malformation of the rats were observed,and the clinical scores and incidence of arthritis were evaluated. On the 19 thday,micro-computed tomography and bone metrology were performed,and histopathological examination of inflammatory joints was performed, andsynovial inflammation,vasospasm,cartilage erosion and bone destruction by pathological severity scores,serum interleukin-1β( IL-1β),tumor necrosis factor-α( TNF-α), vascular endothelial growth factor( VEGF), matrix metalloproteinase-3( MMP-3) and receptor activator of nuclear factor kappa B ligand( RANKL) were detected by enzyme linked immunosorbent assay. Result: Fengshi Qutong capsule could improve the symptoms of inflammatory joint redness,swelling and deformity in CIA rats in a dose-dependent manner. Compared with normal group,clinical score and incidence,joint synovial inflammation,vasospasm,cartilage erosion and pathological score of bone destruction in joint group were significantly increased( P < 0. 01),bone mineral density( BMD),bone volume/total volume( BV/TV),trabecular thickness( Tb. Th),trabecularnumber( Tb. N) were significantly decreased( P < 0. 01),bone surface area/bone volume( BS/BV) and trabecular separation( Tb. Sp) were significantly elevated( P < 0. 01),and levels of IL-1β,TNF-α,VEGF,MMP-3 and RANKL in serum were increased( P < 0. 01);compared with model group,clinical scores an

关 键 词：风湿祛痛胶囊 胶原诱导性关节炎 病理学 影像学 细胞因子 

分 类 号：R289[医药卫生—方剂学] R289.5[医药卫生—中药学]