菜用豌豆品种资源萌发期耐旱性鉴定  

Germplasm Identification of Drought Tolerance of Vegetable Peas During Seeds Germination Stage

作　　者：赵愉风 周清元[1] 郜欢欢 叶桑 王倩[1] 程闯 郭彦军[1] 崔翠[1] ZHAO Yufeng;ZHOU Qingyuan;GA0 Huanhuan;YE Sang;WANG Qian;CHENG Chuang;GU0Yanjun;CUI Cui(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715)

机构地区：[1]西南大学农学与生物科技学院,重庆400715

出　　处：《核农学报》2019年第1期48-59,共12页Journal of Nuclear Agricultural Sciences

基　　金：国家重点基础研究发展计划"973"项目(2014CB1388061);重庆市科委社会民生创新项目(cstc2015shmszx80026)

摘　　要：为研究菜用豌豆种子萌发期的耐旱性,本试验以31份菜用豌豆品种资源为试验材料,通过2.5%PEG溶液模拟干旱胁迫处理萌发期豌豆种子,统计种子发芽势和发芽率,测定鲜重、干重、根长、芽长等指标,并采用相关性分析、主成分分析、聚类分析、灰色关联度分析及逐步回归分析等方法相结合,对31份菜用豌豆品种资源进行萌发期耐旱性鉴定及耐旱指标筛选。结果表明,2.5%PEG-6000模拟干旱胁迫对菜用豌豆种质资源萌发期各测定指标均有极显著影响;根据综合抗旱系数值(CDC值)、耐旱性度量值(D值)、加权关联度(WDC值)大小对供试种质进行排序,其中耐旱性最强的4个品种依次为16110、16055、16140、16107;耐旱性最弱的3个品种依次为16106、16175、16177。通过聚类分析,在欧氏距离D^2=8.5处将31份豌豆品种资源划分为4种类型:第Ⅰ类包括16054、16142等20个品种;第Ⅱ类包括16140、16079和16055 3个品种;第Ⅲ类包括16175、16104等6个品种;第Ⅳ类包括16110和16107 2个品种。逐步回归分析表明,与D值密切相关的指标为发芽指数、活力指数、发芽势、根长,这些可作为豌豆种质资源萌发期耐旱性鉴定综合选择指标。本试验结果为进一步研究菜用豌豆萌发期耐旱性机理及干旱调控缓解机制提供了理论参考,也为菜用豌豆耐旱品种选育及其推广应用奠定了基础。In order to study the drought tolerance of vegetable pea seeds during germination period, 31 vegetable pea varieties were used as experimental materials and were treated with 2.5% PEG-6000 solution to simulate drought stress. The seed germination energy(GE) and germination rate(GR) were counted, and fresh weight(FW), dry weight(DW), root length(RL), buds length(BL) and storage material transfer rate(STR) were measured. Then, correlation analysis, principal component analysis, cluster analysis, grey correlation analysis and stepwise regression analysis were used to identify drought tolerance and screen drought tolerance index of 31 vegetable pea varieties during seeds germination stage. The results showed that 2.5% PEG had significant effect on indexes measured during germination of vegetable pea. The germplasm were sorted according to the comprehensive drought resistance coefficient value(CDC), drought tolerance evaluation value(D) and weighted drought tolerance coefficient value(WDC). As a result, there were 4 varieties including 16110, 16055, 16140 and 16107 showed stronger drought tolerance while 3 varieties including 16106,16175 and 16177 showed drought sensitivity. By cluster analysis, 31 pea germplasm can be divided into 4 group at Euclidean distance D^2=8.5, in which the first group includes 20 varieties such as 16054, 16142, 16138 and so on, the second group includes 16140, 16079 and 16055, the third group contains 6 varieties, such as 16175,16104 and etc, and the fourth group contains 16110 and 16107. Also, stepwise regression analysis showed that the indexes closely related to D value were germination index, vigor index, germination potential and root length, which could be used as indicators of drought tolerance of pea germplasm resources during germination period. The results provide a theoretical reference for further study on drought tolerance mechanism and drought mitigation mechanism of vegetable pea during germination, and laid a foundation for breeding and popularizing the drought-tolerant varie

关 键 词：菜用豌豆 萌发期 耐旱指标 耐旱性鉴定 

分 类 号：S643.3[农业科学—蔬菜学]