海洋真菌Alternaria alternate YX-25和放线菌Streptomyces exfoliates YX-32共培养的次级代谢产物研究  

Secondary metabolites of co-culture of Alternaria alternate YX-25 and Streptomyces exfoliatus YX-32

作　　者：贺小平 粟登权 丁立建[1] 何山[1] HE Xiao-ping;SU Deng-quan;DING Li-jian;HE Shan(Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University,Ningbo 315800,China)

机构地区：[1]宁波大学李达三叶耀珍伉俪李本俊海洋生物医药研究中心,浙江宁波315800

出　　处：《中草药》2018年第24期5772-5779,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(41776168);国家自然科学基金资助项目(41706167);浙江省教育厅科研项目(Y201737271);宁波大学科研基金资助项目(XYL17016)

摘　　要：目的研究来源于福建云霄红树林底泥中的链格孢霉属真菌AlternariaalternateYX-25和链霉菌属放线菌Streptomyces exfoliatus YX-32共培养产生的次级代谢产物。方法通过硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及半制备HPLC等色谱技术对共培养所产生的次级代谢产物进行分离纯化。采用MS和NMR等波谱方法及文献对比鉴定化合物的结构。结果从2株共培养的发酵产物中分离得到9个化合物,分别鉴定为gliotoxin(1)、12,13-dihydroxy-fumitremorgin C(2)、fumitremorgin(3)、bisdethiobis (methylthio) gliotoxin(4)、demethoxyfumitremorgin C(5)、vermistatin(6)、cyclo-(L-Trp-L-Pro)(7)、6-methoxyspirotryprostatin B(8)和citrinolactone D(9)。结论首次从海洋真菌Alternaria alternate YX-25和放线菌Streptomyces exfoliatus YX-32共培养发酵产物中发现了9个化合物,为吲哚二酮哌嗪类、聚酮类、环肽类化合物。共培养方法可使代谢产物丰富,可作为一种诱导次级代谢产物的新方法。Objective To study the secondary metabolites of the co-culture of fungus Alternaria alternata YX-25 and Streptomycesexfoliatus YX-32 isolated from mangrove mud in Yunxiao Country of Fujian Province. Methods The compounds were purified by silica gel and Sephadex LH-20, followed by semi-preparative chromatography. The structures of the compounds were determined by MS and NMR. Results Nine compounds were isolated from the crude extract, which were identified as gliotoxin(1), 12,13-dihydroxy-fumitremorgin C(2), fumitremorgin(3), bisdethiobis(methylthio)gliotoxin(4), demethoxyfumitremorgin C(5), vermistatin(6), cyclo-(L-Trp-L-Pro)(7), 6-methoxyspirotryprostatin B(8), and citrinolactone D(9). Conclusion Nine compounds were isolated for the first time from co-culture of the fungus Alternaria alternata YX-25 and Streptomyces exfoliates YX-32, including indole diketopiperazines, polyketides, and cyclopeptides. The co-cultivation method can be as a new method for enrichment of secondary metabolites.

关 键 词：红树林真菌 次级代谢产物 海洋真菌 放线菌 GLIOTOXIN vermistatin 

分 类 号：R284.1[医药卫生—中药学]