基于腺病毒介导的PD-1干扰对艾灸调控实验性类风湿性关节炎大鼠TCR信号通路Lck、Fyn表达的影响  

Influence of Moxibustionon Expressions of Lck and Fyn of TCR Signal Pathway of RA Rats by Adenovirus Vector-MediatedPD-1 Intervention

作　　者：赖德利 周海燕[1] 陶渊[1] 刘旭光[1] LAI Dell;ZHOU Haiyan;TAO Yuan;LIU Xuguang(Acupunture and Moxibustion College,Chengdu University of TCM,Chengdu 610075,Siehuan,China)

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《中华中医药学刊》2019年第1期45-48,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81403480)

摘　　要：目的:基于腺病毒载体介导的RNAi在体阻断PD-1在类风湿性关节炎(Rheumatoid arthritis,RA)大鼠模型的表达,研究艾灸对实验性RA大鼠TCR信号通路上Lck、Fyn激酶的影响及其PD-1调控机制。方法:采用弗氏完全佐剂对模型组、治疗组和PD-1干扰组进行造模。采用腺病毒载体介导的ShRNA-rPD-1进行基因干扰。采用麦粒灸"肾俞""足三里"两穴结束。采集大鼠脾脏组织,免疫组化法检测各组脾脏组织中Fyn、Lck的表达情况。结果:与对照组比较,模型组大鼠脾脏组织中Lck、Fyn的表达量明显升高表现(P<0.01);与模型组比较,治疗组大鼠Lck、Fyn的表达量均明显低于模型组(P<0.01);与治疗组比较,PD-1干扰组大鼠脾脏组织Lck表达降低不如治疗组(P<0.01),Fyn表达有上升趋势,组间比较无明显差异(P>0.05)。结论:可明显降低TCR信号通路信号分子Lck、Fyn的表达,但在PD-1被干扰的情况下,其抑制Lck、Fyn表达的能力下降,表明该作用可能与PD-1对T细胞活化的负性调控作用相关。Objective: To study the effects of moxibustion on expressions of Lck and Fyn in TCR signal pathway as well as PD-1 regulatory mechanism by adenovirus vector-mediated RNAi in vivo blocking PD-1 expression in RA animal model.Methods:We established AA model by subcutaneous of rats right foot with completed Freunds' Adjuvant(CFA)and used RNAi to get PD-1 gene interference model.Moxa-cone moxibustion treatment was givento Shenshu(BL23),Zusanli(ST36).All rats' foot swelling degrees were observed regularly.Lck and Fyn samples of splenic tissues were examined by immunohistochemistry.Results:Moxibustion could significantly reduce the experimental RA rats' joint perimeters.The role significantly reduced in the case of PD-1 disturbed.Test of Lck, Fyn levels in spleen tissues showed as compared with those from control group, the levels of Lck and Fyn from the RA models elevated significantly(P<0.01);compared with the model group, levels from moxibustion group dramatically reduced(P<0.01);compared with the moxibustion group,the content of Lck in PD-1 RNAi reduced and was less than that of the moxibustion group.Conclusions:Moxibustion could have explicit anti-inflammatory effects and the role of regulating the overactivation of T cells.In the case of PD-1disturbing,the roles can be reduced, which indicated that moxibustion effects are closely related with PD-1 negative regulation on T cell activation.

关 键 词：艾灸 类风湿性关节炎 PD-1 基因干扰 LCK FYN 

分 类 号：R245.81[医药卫生—针灸推拿学] R593.22[医药卫生—中医临床基础]