龙血竭缓释滴丸在家兔体内药动学研究  

Study on Pharmacokinetics of Dragon’s Blood Sustained Release Dropping Pills in Rabbits

作　　者：张晓燕[1] 于莹[1] 徐峰[1] 马伟[1] 董培良[1] 王建明[1] 严广利 ZHANG Xiaoyan;YU Ying;XU Feng;MA Wei;DONG Peiliang;WANG Jianming;YAN Guangli(Research Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学中医药研究院

出　　处：《辽宁中医药大学学报》2019年第1期54-58,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：黑龙江中医药大学优秀青年教师科技创新人才支持计划项目(051241);黑龙江省教育厅科学技术研究项目(12521493)

摘　　要：目的:以龙血素B为检测指标,研究龙血竭在体内药动学参数。方法:以龙血素B为对照品,采用HPLC法测定两组家兔血浆中龙血素B的浓度。同时选择3P97软件进行处理。结果:与市售的广西龙血竭粉末相比,自制龙血竭缓释滴丸的达峰时间明显推迟,且达峰浓度明显降低(P<0.05);但两制剂的药时曲线下面积(AUC),总清除率(CL)以及迟滞时间(Lag time)无显著差异(P>0.05),相对生物利用度F=AUCT·DR/(AUCR·DT)×100%=101.53%。自制的广西龙血竭缓释滴丸符合权重W=1/C^2的单室模型分布,具有较好的缓释效果且吸收程度与普通广西龙血竭粉末等效。结论:建立了用高效液相色谱法测龙血竭缓释滴丸中龙血素B含量的方法,得出了以龙血素B含量为主的缓释滴丸在家兔体内的药动学参数,为进一步深入研究两者剂型的生物等效性和药效学考察提供了实验依据。Objective:To study the parameters which pharmacokinetics of Dragon's Blood in vivo by using loureirin B as the detection index. Methods:The concentration of loureirin B in plasma of rabbits in two groups was determined by HPLC with loureirin B as the reference substance. At the same time choosing 3 P97 software to deal with. Results:Compared with the commercial Dragon's blood of Guangxi,the peaking time of the homemade Dragon's blood slow-release dropping pills was significantly delayed,and the peak concentration was significantly reduced(P<0.05). However,the area under the drug-time curve of the two preparations(AUC),total clearance(CL)and lag time had no obviously difference(P>0.05). Relative bioavailability F=AUCT·DR/(AUCR·DT)×100%=101.53%. The self-made Guangxi Dragon's blood sustained-release dropping pills conformed to the single-chamber model with a weight of W = 1/C^2,and had a good sustained-release effect,and the absorption degree was equivalent to that of an ordinary Guangxi Dragon's blood. Conclusion:A HPLC method was established to measure the content of loureirin B in the Dragon's blood slow-release dropping pills,and the pharmacokinetics of sustained-release dropping pills with rabbit's sustained release dropping pills was obtained. The study parameters provide experimental evidence for further investigation which the bioequivalence and pharmacodynamics of the two formulations.

关 键 词：广西龙血竭 缓释滴丸 高效液相色谱 龙血素B 药物动力学 

分 类 号：R969.1[医药卫生—药理学]