SIRT1对视网膜色素上皮细胞氧化应激和缺血损伤的保护作用  

The Protective Effects of SIRT1 on Oxidative Stress and Ischemic Injury in RPE Cells

作　　者：张瑞卿 田菁 徐媛媛[1] 郝晔[1] ZHANG Rui-qing;JIAN Jing;XU Yuan-yuan;HAO Ye(Department of Pharmacy,Children's Hospital of Nanjing Medical University ,Nanjing 210008,China)

机构地区：[1]南京医科大学附属儿童医院,江苏南京210008

出　　处：《药物生物技术》2018年第6期476-480,共5页Pharmaceutical Biotechnology

摘　　要：为了探讨SIRT1对视网膜色素上皮(RPE)细胞氧化应激和缺血损伤的保护作用,体外培养大鼠视网膜色素上皮细胞RPE,利用SIRT重组慢病毒载体感染细胞获得过表达SIRT1的RPE细胞(OE-RPE),用MTT实验观察SIRT1在氧化应激模型和缺糖缺氧模型(OGD)中对RPE细胞的保护作用,通过重组慢病毒感染获取过表达SIRT1蛋白的视网膜色素上皮细胞。MTT结果显示,与正常组相比,OE-RPE细胞实验组在RPE细胞氧化应激模型和RPE细胞缺糖缺氧模型(OGD)中受到的细胞毒性显著下降,说明SIRT1在RPE细胞氧化应激和缺血损伤中起到一定的保护作用。To investigate the protective effects of SIRT1 on oxidative stress and ischemic injury in RPE cells,the RPE cells were transfected with lentiviral vectors containing the SIRT1 c DNA. The expression of SIRT1 protein was detected by Western blot. And the protective effects of SIRT1 on oxygen-glucose deprivation( OGD) and H2 O2 induced oxidative stress model in RPE cells were measured by MTT. The OE-RPEs with over-expressed SIRT1 protein was obtained after recombinant lentivirus infection and the cell viability of OE-RPEs treated with OGD and H2 O2 were significantly increased compared with that of the control group. SIRT1 overexpression can protect PRE cells from oxidative stress and ischemic injury.

关 键 词：SIRT1 氧化应激 OGD模型 RPE细胞 

分 类 号：R965[医药卫生—药理学]