过量氟对小鼠骨钙素与糖代谢的影响  

Effects of excessive fluoride on osteocalcin and glucose metabolism in mice

作　　者：秦纹 张美林 陈龙[3] 张美玉 任海洋 钟近洁 Qin Wen;Zhang Meilin;Chen Long;Zhang Meiyu;Ren Haiyang;Zhong Jinjie(Department of Histology and Embryology,Basic Medical College,Xinjiang Medical University,Urumqi 830011, China;Xinjiang Urumqi Blood Center,Urumqi 830011,China;Functional Center,Basic Medical College,Xinjiang Medical University,Urumqi 830011,China;Clinical College,Xinjiang Medical University,Urumqi 830011,China;Zijingang Campus Medical School,Zhejiang University,Hangzhou 310000,China)

机构地区：[1]新疆医科大学基础医学院组织胚胎学教研室,乌鲁木齐830011 [2]新疆乌鲁木齐市血液中心,830011 [3]新疆医科大学基础医学院机能中心,乌鲁木齐830011 [4]新疆医科大学临床学院,乌鲁木齐830011 [5]浙江大学紫金港校区医学院,杭州310000

出　　处：《中华地方病学杂志》2019年第1期8-11,共4页Chinese Journal of Endemiology

基　　金：新疆维吾尔自治区科技厅项目(2017D01C190).

摘　　要：目的 观察过量氟对小鼠骨钙素(OCN)与糖代谢的影响。 方法 选用32只C57雄性成年小鼠(体质量为18-24 g),按体质量采用随机数字表法分为4组:0(对照)、50、100、150 mg/L染氟组,每组8只,分别自由饮用含0、50、100、150 mg/L氟化钠的蒸馏水,以复制饮水型氟中毒动物模型。12周后采用酶联免疫吸附实验(ELISA)检测血清中总骨钙素(tOCN)、未羧化骨钙素(ucOCN)、胰岛素(FINS)和胰高血糖素;化学发光法检测空腹血糖(GLU)、糖化血红蛋白(HbA1c)含量。 结果 染氟12周后各组小鼠血清tOCN、ucOCN、GLU、HbA1c、FINS、胰高血糖素含量比较,差异有统计学意义(F = 17.23、22.29、4.43、45.57、4.45、55.21,P均< 0.05)。其中100、150 mg/L染氟组小鼠血清tOCN、ucOCN、GLU、HbA1c、FINS含量[(30.02 ± 5.35)、(35.22 ± 4.98)μg/L,(8.72 ± 1.34)、(11.01 ± 1.02)μg/L,(7.53 ± 2.29)、(8.53 ± 2.81)mmol/L,(6.74 ± 0.68)、(7.12 ± 0.25)mmol/L,(2.65 ± 0.25)、(2.74 ± 0.47)mU/L]均高于对照组[(20.23 ± 3.22)、(5.80 ± 1.60)μg/L,(4.99 ± 1.60)、(4.95 ± 0.28)mmol/L,(2.13 ± 0.28)mU/L,P均< 0.05],而50、100、150 mg/L染氟组小鼠血清胰高血糖素含量[(20.90 ± 3.00)、(23.68 ± 2.58)、(21.63 ± 2.42)ng/L]低于对照组[(38.61 ± 3.73)ng/L,P均< 0.05]。 结论 过量的氟摄入可导致小鼠血清OCN水平升高及糖代谢异常。Objective To observe the effects of excessive fluoride on osteocalcin and glucose metabolism in mice. Methods Thirty-two male C57 mice (body mass: 18-24 g) were selected as study subjects which were randomized into four groups (8 mice in each group) according to their body mass by random number table method: 0 mg/L group (control group), 50 mg/L fluorine group, 100 mg/L fluorine group and 150 mg/L fluorine group. Sodium fluoride in distilled water was freely taken by these animals to replicate fluorosis animal model. After 12 weeks, total osteocalcin, uncarboxylated osteocalcin, insulin and glucagon were measured by enzyme-linked immunosorbent assay, fasting blood glucose and glycated hemoglobin were measured by chemiluminescence. Results After 12 weeks of intervention with sodium fluoride, serum total osteocalcin, uncarboxylated osteocalcin, fasting blood glucose, glycated hemoglobin, insulin, and glucagon were significantly different between different groups (F = 17.23, 22.29, 4.43, 45.57, 4.45, 55.21, P < 0.05). Total osteocalcin, uncarboxylated osteocalcin, fasting blood glucose, glycated hemoglobin, and insulin in the 100, 150 mg/L fluorine groups were higher than those of control group [(30.02 ± 5.35), (35.22 ± 4.98) vs (20.23 ± 3.22) μg/L;(8.72 ± 1.34), (11.01 ± 1.02) vs (5.80 ± 1.60) μg/L;(7.53 ± 2.29), (8.53 ± 2.81) vs (4.99 ± 1.60) mmol/L;(6.74 ± 0.68), (7.12 ± 0.25) vs (4.95 ± 0.28) mmol/L;(2.65 ± 0.25), (2.74 ± 0.47) vs (2.13 ± 0.28) mU/L, P < 0.05]. The serum glucagon levels in the 50, 100, 150 mg/L fluorine groups were lower than that in the control group [(20.90 ± 3.00), (23.68 ± 2.58), (21.63 ± 2.42) vs (38.61 ± 3.73) ng/L, P < 0.05]. Conclusion Excessive fluoride can lead to elevated osteocalcin level and abnormal glucose metabolism in mice.

关 键 词：氟化物 骨钙素 糖代谢 

分 类 号：R599.1[医药卫生—内科学]