利拉鲁肽对急性心肌梗死后再灌注大鼠左心室电活动稳定性质的影响  

Effect of Liraglutide on Left Ventricular Electrical Stability after Acute Myocardial Ischemia-Reperfusion Injury in Rats

作　　者：王阿妮[1] 金莉子[1] 马英东[2] 卢慧芳[1] 董杰 王梁 林岫芳[1] WANG A-ni;JIN Li-zi;Ma Ying-dong;LU Hui-fang;DONG Jie;WANG Liang;LIN Xiu-fang(Department of Cardiology,The Fifth Affiliated Hospital of Sun Yat-sen University,519000)

机构地区：[1]中山大学附属第五医院心血管内科,519000 [2]中山大学附属第五医院介入医学科,519000

出　　处：《岭南急诊医学杂志》2018年第6期514-517,共4页Lingnan Journal of Emergency Medicine

基　　金：广东省医学科学技术研究基金项目资助(A2016341)

摘　　要：目的:旨利用利拉鲁肽对急性心肌梗死后(简称心梗)再灌注大鼠进行预处理,观察利拉鲁肽对再灌注前后大鼠心室电活动不稳定性的影响。方法:将健康成年雄性大鼠24只随机分为三组:正常对照组(NC组,n=8)、安慰剂组(PB组,n=8)、利拉鲁肽组(LIR组,n=8)。NC组不做处理;PB组、LIR组分别给予腹腔皮下注射生理盐水、利拉鲁肽200μg/(kg·d),一天两次。注射7天后,NC组只做观察,PB组和LIR组通过结扎前降支动脉制备急性心梗模型。随后开通结扎的前降支动脉,超声心动图测量左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)和左心室射血分数(LVEF);随后通过S1S2程序性刺激评估三组大鼠左心室有效不应期(LVERP)。实验结束前,测定梗死面积及TNF-α。最后利用S1S1连续电刺激测定室颤阈值(VFT)。结果:与NC组相比,PB组的LVERP明显缩短(P<0.01),LIR组的LVERP缩短,但无统计学差异(P>0.05);与PB组相比,LIR组的LVERP明显增加(P<0.05)。与NC组相比,PB、LIR组的VFT均明显降低(P<0.01);与PB组相比,LIR组的VFT明显升高(P<0.01)。与PB组相比,LIR组的LVEDD、LVESD明显减小(P<0.01),LVEF明显增加(P<0.05)。与PB组相比,LIR组的梗死面积明显减小(P<0.05),且TNF-α表达显著减少(P<0.05)。结论:利拉鲁肽可以明显减少心梗后再灌注大鼠左心室的电活动不稳定性,同时改善了左心室重构,减少了炎症因子的表达及梗死面积,进而降低了心梗后再灌注损伤。Objective: To investigate the effect of liraglutide on the left ventricular electrical stability after acute myocardial ischemia-reperfusion injury(IRI)in rats. Methods:Twenty-four healthy adult male rats were randomly divided into three groups:NC group(n=8),PB group(n=8),and LIR group(n=8). The NC group was not treated,the PB and LIR group were under subcutaneous injection of normal saline and liraglutide 200 μg/(kg·d)twice a day respectively for seven days. Then,all the rats were established by ligating the rat anterior descending artery,except the NC group. The left ventricular end-diastolic period was LVEDD,LVESD and left LVEF were measured by echocardiography;The LVERP,VFT were recorded via S1S2 and S1S1 programmed stimulation;HE and TTC staining were used to observe the left ventricular myocardial cell size. Immunohistochemical analysis of TNFα was applied to evaluate the cardiac inflammatory response. Results:Compared with NC group,the LVERP of PB group was significantly shortened(P<0.01),but the LVERP of LIR group was no statistical difference(P>0.05). Compared with PB group,the LVERP was significantly increased in LIR group(P<0.01). Compared with NC group,the VFT of PB and LIR groups were significantly decreased(P<0.01);compared with PB group,the VFT of LIR group was significantly increased(P<0.01). Compared with NC group,the LVEDD and LVESD of PB and LIR groups were significantly increased(P<0.05),while theLVEF was significantly decreased(P<0.05). Compared with PB group,the LVEDD and LVESD were significantly de-creased(P<0.05)and LVEF was significantly increased(P<0.05)in LIR group. Compared with PB group,the infarctsize of the LIR group was significantly reduced(P<0.05),and expression of TNF-α was significantly decreased(P<0.05). Conclusion:Liraglutide can significantly reduce the instability of left ventricular electrical activity in rats afterAMI,improve left ventricular remodeling and reduce the expression of TNF-α and infarct size,then reduce myocardialischemia-reperfusion injury.

关 键 词：心血管病学 急性心肌梗死后再灌注 利拉鲁肽 心室电活动 

分 类 号：R542.22[医药卫生—心血管疾病]