^(18)F-FDG PET/CT显像评价二甲双胍对大鼠心肌缺血再灌注损伤的保护作用  

^(18)F-FDG PET/CT imaging evaluating the effect of metformin on myocardial ischemia/reperfusion injury in rats

作　　者：苏航[1] 田毅 朱紫薇 李珺奇[1] 杨子鹤 常智 李全[1] 牟甜甜[1] 李翔 张晓丽[1] SU Hang;TIAN Yi;ZHU Ziwei;LI Junqi;Yang Zihe;CHANG Zhi;LI Quan;MOU Tiantia;LI Xiang;ZHANG Xiaoli(Department of Nuclear Medicine,Bering Anzhen Hospital,Capital Medical University, Bering Institute of Heart,Lung and Blood Vessel Diseases,Beijing 100029,China)

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所核医学科,100029 [2]维也纳医科大学维也纳总医院核医学科

出　　处：《心肺血管病杂志》2018年第12期1125-1128,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：国家自然基金资助项目(81571717);首都临床特色应用研究(2181100001718071)

摘　　要：目的:采用^(18)F-FDG microPET/CT显像,探讨二甲双胍(MF)干预治疗对大鼠心肌缺血再灌注损伤的保护作用。方法:选用雄性,SD大鼠,12只,于左心耳下靠肺动脉圆锥处结扎冠状动脉左前降支,建立缺血再灌注损伤模型。随机分为MF干预组(n=6)及正常对照组(n=6),其中MF干预组给予50mg·kg^(-1)·12h^(-1)剂量MF灌胃,正常对照组给予等体积0.9%氯化钠溶液灌胃。分别于再灌注后1d、7d、14d、30d行^(18)F-FDG micro PET/CT门控心肌代谢显像。于大鼠心肌缺血中心区、边缘区、远端区勾画容积感兴趣区(VOI),计算不同部位^(18)F-FDG的标准摄取最大值(SUVmax),计算TBR值=缺血中心区SUVmax/远端区SUVmax。分别勾画左心室舒张末期容积(EDV, mm^3)及收缩末期容积(ESV, mm^3),计算LVEF。结果:对照组,TBR值从再灌注后1～30d逐渐减低,EDV逐渐增大,两者呈负相关关系(r=-0.732,P=0.000);在MF干预组中,TBR值随时间延长无明显变化趋势。再灌注第30d,TBR值,MF干预组较对照组明显升高[(0.81±0.06)vs.(0.65±0.09),P<0.05],MF干预组的LVEDV明显低于对照组[(358.21±22.62)vs.(457.53±29.91),P<0.05]。LVEF在再灌注不同时间点,两组差异无统计学意义。结论:^(18)F-FDG PET/CT显像可以动态评估大鼠心肌缺血再灌注损伤随时间变化的规律。MF干预对缺血再灌注损伤后有一定的保护作用,并可以延缓左心室重构的发生和发展。Objective: To evaluate the effect of metformin(MF) on ischemia/reperfusion(I/R) injury models in rat at different time points after I/R injury by 18F-fluorodeoxyglucose(18F-FDG) micro PET/CT imaging. Methods: SD rats(n=12) were randomly divided into 2 groups: MF intervention group(n=6), MF(50 mg/kg) was administrated orally twice per day and control group(n=6). Left anterior descending(LAD) coronary artery was ligated near the pulmonary arch root for 30 mins, to establish an I/R injury model. Gated18F-FDG micro PET/CT imaging was acquired at different time points(day 1, days 7, day 14 and day 30 after reperfusion). Volume of interests(VOIs) was drawn in different regions(ischemia center, peri-ischemia area and remote area). Standardized uptake values(SUVmax) were calculated to evaluate the glucose metabolism, and the LVEDV and end systolic volume(ESV) were calculated to evaluate the LVEF. Finally, the rats were scarified to observe the pathological changes of myocardial tissue. TBR value was calculated by SUVmax in ischemia center/SUVmax in remote region. Results: In control group, TBR value was gradually reduced from day 1 to day 30 after reperfusion. In the meantime, LVEDV was gradually increased with time prolonged. TBR value was negatively correlated with LVEDV(r=-0.732,P<0.05). In MF intervention group, TBR value didn’t change significantly(P>0.05. On day 30 after reperfusion, TBR in metformin intervention group was significantly higher than those in control group [(0.81±0.06) vs.(0.65±0.09), P<0.05]. LVEDV in MF intervention group were significantly lower than in control group [(358.21±22.62) vs.(457.53±29.91), P<0.05], LVEF did not differ between MF and control groups at different time points after reperfusion(P>0.05). Conclusions: MF may protect the I/R injury myocardium and attenuate the extent of left ventricular enlargement.18 F-FDG micro PET/CT imaging was an effective technique to evaluate the effect of MF on myocardial I/R injury in rats.

关 键 词：缺血再灌注损伤 二甲双胍 PET/CT ^18F-FDG 

分 类 号：R54[医药卫生—心血管疾病]