白薇生物碱类成分抑制柑橘采后青霉病菌活性  

Antifungal activity of Cynanchum atratum alkaloids against citrus postharvest blue mould

作　　者：陈楚英 彭旋 陈金印 万春鹏 CHEN Chuying;PENG Xuan;CHEN Jinyin;WAN Chunpeng(College of Agronomy,Jiangxi Agricultural University/Jiangxi Key Laboratory for Post-harvest Technology and Nondestructive Testing of Fruits &Vegetables/Collaborative Innovation Center of Post-harvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Nanchang 330045,Jiangxi,China;Pingxiang University,Pingxiang 337055,Jiangxi,China)

机构地区：[1]江西农业大学农学院.江西省果蔬保鲜与无损检测重点实验室.江西省果蔬采后处理关键技术与质量安全协同创新中心,南昌330045 [2]萍乡学院,江西萍乡337055

出　　处：《果树学报》2019年第1期94-102,共9页Journal of Fruit Science

基　　金：国家自然科学基金(31760598)

摘　　要：【目的】探究白薇抑制柑橘采后青霉病菌——意大利青霉活性部位的化学成分。【方法】采用抑菌活性靶向分离、溶剂萃取、各种柱层析及制备液相色谱分离单体化合物,核磁共振(NMR)和高分辨离子肼质谱(HR-IT-TOF-MS)鉴定分离的化合物。【结果】白薇乙酸乙酯(CAE)和正丁醇(CAB)萃取物有抑菌活性,正丁醇萃取物抑菌活性最强,大孔树脂进一步分离富集抑菌活性,CAB-C2为白薇抑菌活性部位,质量浓度为0.625 mg·mL-1时其抑菌圈直径达到16.25 mm。采用各种柱层析及制备液相色谱技术从活性部位中分离出5个单体化合物,经NMR和MS鉴定为10β-N-氧化-7-脱甲氧基娃儿滕碱(1)、9-脱氢安托芬(2)、9,14-脱氢安托芬(3)、14羟基-N-氧化-7-脱甲氧基娃儿滕碱(4)、10α-N-氧化-7-脱甲氧基娃儿滕碱(5)。【结论】5种生物碱类活性成分均为首次从白薇中分离鉴定,生物碱类成分为白薇抑制柑橘采后青霉病菌的主要活性成分。[Objective]Citrus fruits are often subjected to many postharvest diseases caused by various pathogens during picking,storage and transportation.Green and blue molds,caused by Penicillium digitatum and P.italicum,respectively,are two major postharvest citrus diseases and cause significant economic losses in the commercialization phase.Currently,control of postharvest citrus diseases relies mainly on the use of synthetic fungicides,which usually result in fungicide-resistance of the fungi,environment pollution and food safety problem.In recent years,much attention has been given to preventing citrus from the diseases by natural edible plant extracts.Scientists worldwide devote their time and energy to discover the high effect,low toxicity,safe and inexpensive plant-derived fungicides.Our researches also focus on plant-derived fungicides and chemical constituents target to inhibit P.digitatum and P.italicum in vitro and in vivo.Previously we showed the antifungal activities of Cynanchum atratum(CA)against 15 phytopathogenic fungi causing the postharvest diseases in fruits and vegetables.However,antifungal compoments were not clear.The extracts of n-butanol showed the best antifungal activity against P.italicum.In order to illuminate the antifungal constituents of the active fraction from C.atratum(CA)against phytopathogenic fungi P.italicum that cause citrus postharvest blue mould.[Methods]Antifungal bioassay-guided isolation and identification of constituents in the active fractionfollowed by liquid-liquid extraction and various column chromatographies.The preparative HPLC was used finally to get the pure compounds.The isolated compounds were identified by NMR and HR-ITTOF-MS.[Results]Ethyl acetate(CAE)and n-butanol(CAB)liquid-liquid extracts at the concentration of 25 mg· mL^-1 showed antifungal activity with the inhibition zone of 14.3 and 20.5 mm,respectively.Moreover,CAB showed a higher antifungal activity than CAE and CA at the same concentrations.The water extracts showed no antifungal activity.CAB was furth

关 键 词：柑橘:青霉病 意大利青霉 白薇 生物碱 活性成分 

分 类 号：S666.2[农业科学—果树学]