解毒通络及养阴中药处方对人胃癌细胞BGC-823的影响  

Effect of Chinese Prescriptions of Removing Toxicity and Dredging Collateral as well as Nourishing Yin on Human Gastric Cancer Cell BGC-823

作　　者：申小红 孙媛媛 程光 Shen Xiaohong;Sun YuanYuan;Cheng Guang(Affiliated Hospital of North China University of Science and Technology,Tangshan China,063000;North China University of Science and Technology,Tangshan China,063000)

机构地区：[1]华北理工大学附属医院,河北唐山063000 [2]华北理工大学,河北唐山063000

出　　处：《陕西中医药大学学报》2019年第1期86-90,共5页Journal of Shaanxi University of Chinese Medicine

摘　　要：目的研究中药解毒通络及养阴处方对人胃癌细胞BGC-823的影响。方法通过小鼠灌胃给药获取血清经过无菌处理后干预胃癌细胞BGC-823,使用细胞增殖实验、Western blot实验检测细胞增殖、凋亡以及侵袭力的变化。结果细胞增殖试验显示,生理盐水组较空白对照组细胞增殖力无明显变化P> 0. 05,解毒通络及养阴组较生理盐水组细胞增殖力明显降低P <0. 05,Western blot结果显示,解毒通络和养阴组较生理盐水组,ERK、MMP9蛋白表达明显降低(P <0. 05),Bax蛋白表明显增强(P <0. 05),养阴组较生理盐水组,E-Cad蛋白表量明显增强(P <0. 05)。结论解毒通络及养阴中药处方能够影响ERK、MMP9、BAX、E-Cad蛋白表达水平,抑制胃癌细胞BGC823增殖,促进其凋亡,能够在一定程度上起到抗癌作用。Objective: To investigate the effect of Chinese prescriptions of removing toxicity and dredging collateral as well as nourishing yin on human gastric cancer cell BGC-823. Method: Serum was obtained by intragastric administration in mice and sterilized to intervene in gastric cancer cell BGC-823. Cell proliferation,apoptosis and invasiveness were detected by cell proliferation test and Western blot test. Result: Cell proliferation test showed that there was no significant change in cell proliferation in normal saline group compared with blank control group( P>0.05). Cell proliferation in removing toxicity and dredging collateral group and nourishing yin group were significantly lower than that in normal saline group( P<0.05). Western blot results showed that the expressions of ERK and MMP9 protein in removing toxicity and dredging collateral group and nourishing yin group were significantly lower than that in normal saline group( P<0.05). Bax protein expression showed a significant increase( P<0.05). Compared with saline group,E-Cad protein expression increased significantly in nourishing yin group( P<0.05). Conclusion: Chinese prescriptions of removing toxicity and dredging collateral as well as nourishing yin can affect the expression levels of ERK,MMP9,BAX and E-Cad proteins,inhibit the proliferation of gastric cancer cells BGC823,promote their apoptosis,and play an anti-cancer effect to a certain extent.

关 键 词：BGC-823 ERK MMP9 解毒通络 益气养阴 

分 类 号：R285.5[医药卫生—中药学]