2015-2017年贵州省威宁13株H9N2亚型禽流感病毒HA基因序列分析  

Sequence analysis of HA genes of 13 H9N2 AIV,Weining,2015-2017

作　　者：万永虎[1] 雷强 赵德恩 谢江松 唐光鹏[1] 庄丽[1] 郑勤妮[1] 任丽娟[1] 杨桃梅 李世军[1] WAN Yong-hu;LEI Qiang;ZHAO De-en;XIE Jiang-song;TANG Guang-peng;ZHUANG Li;ZHENG Qin -ni;REN Li -juan;YANG Tao -mei;LI Shi -jun(Center for Disease Control and Prevention of Guizhou,Guiyang,Guizhou 550004,China)

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004 [2]威宁县疾病预防控制中心,贵州威宁553100 [3]贵州医科大学,贵州贵阳550004 [4]山东大学,山东济南250012

出　　处：《现代预防医学》2019年第3期537-542,共6页Modern Preventive Medicine

基　　金：贵州省卫生计生委科学技术基金项目(gzwjkj2016-1-056);贵州省重要传染病实验诊断技术及分子流行病学研究科技创新人才团队(黔科合人才(2018)5606);贵州省高层次创新型人才培养项目(黔科合人才(2016)4021号)

摘　　要：目的了解2015-2017年贵州省威宁H9N2亚型禽流感病毒的分子特征,为禽流感病毒的研究与防控提供依据。方法对选取的13株病毒进行核酸的提取、血凝素基因(hemagglutinin,HA)的扩增和测序,运用生物信息学软件对H9N2禽流感病毒(AIV) HA基因的同源性、遗传进化、受体结合关键位点、致病相关位点和糖基化位点变异情况进行分析。结果 13株H9N2AIV的HA基因核苷酸和氨基酸同源性分别为96. 1%～99. 9%和95. 7%～100%,均属于DK/HK/Y280/97分支。HA基因与致病性高低相关的裂解位点区域2株为PSRSNR↓GLF,11株为PSRSSR↓GLF,均属于低致病性毒株。与传播感染相关的受体结合位点均发生E198T和Q234L的突变,具有人样受体结合特征。13株毒株HA均含7个与毒力相关的糖基化位点,218位点均存在一个糖基化位点缺失,313位点均存在一个糖基化位点的增加。结论贵州省威宁县H9N2AIVs属于DK/HK/Y280/97系,均为低致病性禽流感病毒,存在人易感位点,病毒一直处于不断的变异之中,故应加强其监测与防控。Objective To understand the molecular characteristics of the hemagglutinin gene( HA) of H9N2 subtype avian influenza virus( AIV) in Weining from 2015 to 2017,and to provide scientific evidence for the prevention and control of AIV. Methods RNAs were extracted and the HA genes from the 13 samples with H9N2 positive screened by Real-Time PCR of live poultry market( LPM) environment were sequenced. Then the homology,genetic evolution,pivotal sites related to receptor binding regions,pathogenicity and potential glycosylation of H9N2 subtype AIV were analyzed by a series of bioinformatic softwares. Results 96. 1% ~ 99. 9% and 95. 7% ~ 100% strains were similar among 13 strains in nucleotide and amino acid of the HA gene,respectively. All strains belonged to Eurasian lineage,DK/HK/Y280/97 sublineage. The cleavage sites motif of2 strains was PSRSNR↓GLF and that of 9 strains was PSRSSR↓GLF,both belonging to low pathogenic strains. Mutations in E198 T and Q234 L at the receptor binding sites in the HA were found in All strains indicated the potential of binding with the human-like receptor. All 13 strains had 7 glycosylation sites,a site deletion at amino acid site 218,and addition at313. Conclusion All H9N2 strains belong to DK/HK/Y280/97 sublineage,and they are low pathogenic avian influenza viruses in Weining,but mutations of receptor binding site may enhance the susceptibility and pathogenicity to human beings,and H9N2 virus is continual changing. Hence,enhancing the surveillance and control of H9N2 virus is necessary.

关 键 词：H9N2禽流感病毒 血凝素基因 分子特征 

分 类 号：R181.33[医药卫生—流行病学]