日本蛇根草花青素还原酶基因的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of ANR Gene in Ophiorrhiza japonica

作　　者：孙威[1] 申欢 陈婷[1] 彭贵 潘蓉蓉 鞠志刚 Sun Wei;Shen Huan;Chen Ting;Peng Gui;Pan Rongrong;Ju Zhigang(Key Laboratory of Plant Physiology and Development Regulation,College of Life Science,Guizhou Normal University,Guiyang,550025;Medicine College,Guiyang University of Chinese Medicine,Guiyang,550025)

机构地区：[1]贵州师范大学生命科学学院植物生理发育调控重点实验,贵阳550025 [2]贵阳中医学院药学院,贵阳550025

出　　处：《基因组学与应用生物学》2019年第1期233-238,共6页Genomics and Applied Biology

基　　金：贵州省联合基金项目(黔科合LH字(2016)7211号);贵州师范大学资助博士科研项目(0516006);贵州省重点实验室建设项目(黔科合计Z字(2011)4005);国家自然科学基金项目(31300317);教育部喀斯特山地生物多样性保护与可持续利用创新团队共同资助

摘　　要：花青素还原酶(anthocyanidin reductase, ANR)是原花青素合成途径中的关键酶,同时花青素还原酶对植物花色苷的积累也发挥着重要作用。本研究以日本蛇根草为材料,以其转录组测序结果为基础,设计引物通过RT-PCR方法成功克隆得到日本蛇根草ANR基因完整的cDNA序列,利用生物信息学方法和工具对日本蛇根草ANR基因的功能结构域、生理和化学参数、亲/疏水性、信号肽、二级结构等进行预测和分析。结果表明,该基因cDNA全长为1 020 bp,编码339个氨基酸,预测其蛋白分子量为36.37 kD,等电点为5.92,为亲水蛋白,不含信号肽序列。综上,本研究成功克隆获得日本蛇根草ANR基因并完成其生物信息学分析,研究结果将为解析该基因的功能奠定基础。Anthocyanidin reductase(ANR) is the key enzyme in the procyanidins biosynthetic pathway, and it also plays an important role in the accumulation of anthocyanin in plants. In this study, we utilized Ophiorrhiza japonica as materials, and designed the primers according to the results of the transcriptome sequencing. The integrated cDNA sequence of ANR gene was cloned successfully by RT-PCR method in Ophiorrhiza japonica. Bioinformatics methods and tools were applied to the functional domains, physical and chemical parameters, hydrophobicity or hydrophilicity, signal peptide, secondary structure and other factors in ANR gene of Ophiorrhiza japonica for prediction and analysis. The results showed that the full length of the gene cDNA was 1 020 bp, which encoded 339 amino acids. The protein molecular weight was predicted to be 36.37 kD and the isoelectric points were 5.92, ANR gene of Ophiorrhiza japonica was a hydrophilic protein with no signal peptide sequence. In summary, we have successfully cloned the ANR gene from Ophiorrhiza japonica and finished its bioinformatics analysis, these results will lay a foundation for the analysis of the function of this gene.

关 键 词：日本蛇根草 ANR 基因克隆 生物信息学分析 

分 类 号：Q943.2[生物学—植物学] S567.239[农业科学—中草药栽培]