胡杨DREB2A和WRKY19基因启动子逆境响应元件及其功能分析  

Stress Response Elements of DREB2A and WRKY19 Gene Promoters in Populus euphratica and Their Functional Analysis

作　　者：王叶利 唐文思 王华芳[1] Wang Yeli;Tang Wensi;Wang Huafang(National Engineering Laboratory for Tree Breeding,College of Biology Sciences and Biotechnology,Beijing Forestry University,Beijing,100083)

机构地区：[1]北京林业大学生物科学与技术学院林木育种国家工程实验室,北京100083

出　　处：《分子植物育种》2019年第2期428-436,共9页Molecular Plant Breeding

基　　金：北京林业大学研究生课程建设项目(YWKC15012);国家自然科学基金项目(31370598)共同资助

摘　　要：胡杨(Populus euphratica Oliv.)是唯一适应沙漠环境的高大乔木树种,抗逆性极强。其抗逆机制对植物抗性育种极为重要,国内外极为关注。本研究以生物信息学和转GUS基因烟草瞬时表达等手段解析胡杨PeDREB2A和PeWRKY19基因启动子序列顺式作用元件。结果表明,两个启动子均有干旱、NaCl、4℃、ABA响应顺式作用元件,CANNTG (N=A/T/C/G)、GRWAAW (R=G/A, W=A/T)、ACGT和CACATG等,增强GUS基因表达。PeDREB2A基因启动子序列1 886 bp,分别含干旱、盐、低温、ABA响应顺式作用元件等9个、11个、9个和5个;-888～-1 271 bp为盐负调控区,-1 271～-1 738 bp为干旱正调控区和ABA负调控区,-1 738～-1 878 bp为盐正调控区和干旱负调控区。PeWRKY19基因启动子序列为1 734 bp,分别含干旱、盐、低温和ABA响应顺式作用元件16个、5个、11个和4个;-176～-417 bp为盐负调控和ABA负调控区,-417～-696 bp为干旱负调控区和低温正调控区,-696～-849 bp为盐正调控区。本研究为阐释胡杨抗逆分子机制提供基础。Populus euphratica is the onl y arbor species adapted to the desert land, which has extremely strong stress resistance. Its resistance mechanism is quite importance for resistance breeding of plant, and it has attracted much attention at home and abroad. In this study, cis-acting elements of promoter sequences of PeDREB2 A and PeWRKY19 genes in Populus euphratica were analyzed by bioinformatics and transient expression of GUS transgenic tobacco. The results showed that these two promoters both had cis-acting elements CANNTG(N=A/T/C/G),GRWAAW(R=G/A, W=A/T), ACGT and CACATG in responding to drought, NaCl, 4℃, and ABA, respectively.They enhanced the expression of GUS gene. PeDREB2 A promoter sequence was 1 886 bp, containing 9, 11, 9 and5 cis-acting elements in responding to drought, salt, low temperature and ABA, respectively. The region rangeed from-888 to-1 271 bp was negative regulation in responding to salt,-1 271 to-1 738 bp was positive regulation to drought and negative to ABA, and-1 738^-1 878 bp was positive regulation to salt and negative to drought.The promoter sequence of PeWRKY19 was 1 734 bp in full length, containing 16, 5, 11 and 4 cis-acting elements in responding to drought, salt, low temperature and ABA, respectively. Its regions ranged from-176 to-417 bp was negative regulation to salt and ABA,-417 to-696 bp was negative to drought but positive to low temperature,and-696 to-849 bp was positive to salt, respectively. The study could provide the foundation for explaining the molecular mechanism of stress resistance in Populus euphratica.

关 键 词：胡杨(Populus euphratica) 启动子 顺式作用元件 DREB2A WRKY19 

分 类 号：S792.11[农业科学—林木遗传育种]