基于抗风湿作用的淫羊藿拆分组分筛选研究  

Screening Research on Split Fractions of Epimedium br evicornu Maxim based on Anti-rheumatism Effect

作　　者：卢芳 张凯 刘树民 LU Fang;ZHANG Kai;LIU Cunmin(Heilongjiang University of Chinese Medicine,Heilongjiang Harbin 150040,China;Center for drug safety evaluation of Heilongjiang University of Chinese Medicine,Heilongjiang Harbin 150040,China)

机构地区：[1]黑龙江中医药大学继续教育学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学研究生院,黑龙江哈尔滨150040 [3]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040

出　　处：《中医药临床杂志》2019年第1期105-110,共6页Clinical Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划973计划课题(2013CB531804);黑龙江中医药大学优秀创新人才支持计划资助

摘　　要：目的:探讨淫羊藿及其拆分组分提取物对佐剂诱导的风湿模型大鼠的影响,从而筛选出抗风湿最有效部位,为开发抗风湿中药新制剂奠定基础。方法:足跖皮内注射完全弗氏佐剂(CFA)建立大鼠佐剂型风湿模型,测量足趾容积,计算足肿胀度。造模后第15d开始灌胃给予淫羊藿全成分及各拆分组分,连续两周,末次给药24h后,测定血清中的IL-1β、IL-6、IL-8、TNF-α、TLR2、TLR4、NF-κB含量,并对大鼠膝关节滑膜进行HE染色,观察其病理变化。结果:从足肿胀结果看出模型组和95醇段给药组在4-28d肿胀体积递增,全成分组在给药1d后肿胀情况开始回调;从血清检测结果看出,全成分组可使血清中IL-1β、IL-8、TNF-α、TLR4含量明显下降(P<0.05),使IL-6含量极为显著性下降(P<0.01);30醇组可显著降低血清中IL-6、IL-1β含量(P<0.01),并对TNF-α、TLR2、NF-κB含量也显著降低(P<0.05);病理切片结果显示,全成分组、30醇组和95醇组均不同程度改善滑膜组织的病理变化。结论:淫羊藿全成分组、30醇组和95醇组对佐剂诱导的风湿模型大鼠有明显的治疗作用。淫羊藿各组分抗风湿作用大小依次为:全成分>30醇段>95醇段>水层段>多糖段,其作用机制可能与改善血液中炎性因子的表达和抑制组织病理变化有关。Objective:To investigate the effects of extracts of Epimedium and its fractions on adjuvant-induced rheumatoid model rats.Screen out the most effective part of anti-rheumatic,and lay the foundation for the development of new anti-rheumatic Chinese medicine preparations.Methods:A rat adjuvanted rheumatoid model was established by intradermal injection of complete Freund's adjuvant (CFA).The toe volume is measured and the degree of swelling of the foot is calculated.On the 15th day after model establishment,the whole component and each split component of Epimedium were administered by gavage for two weeks.The serum levels of IL-1β,IL-6,IL-8,TNF-α,TLR2, TLR4,and NF-κB were measured 24 hours after the last administration.HE staining of rat knee synovium was performed to observe pathological changes.Results:From the results of foot swelling,the swelling volume of the model group and the 95 alcohol group was increased from 4 to 28 days,and the swelling of the whole component group began to be adjusted after 1 day of administration.From the serum test results,the content of IL-1β,IL-8,TNF-α and TLR4 in serum was significantly decreased in the whole component group (P<0.05),and the IL-6 content was significantly decreased (P<0.01).30 Alcohol group can significantly reduc'e the levels of IL-6 and IL-1β in serum (P<0.01). The contents of TNF-α,TLR2 and NF-κB were also significantly decreased (P<0.05).The results of pathological section showed that the whole component group,30 alcohol group and 95 alcohol group improved the pathological changes of synovial tissue to varying degrees.Conclusion:Epimedium group,30 alcohol group and 95 alcohol group have obvious therapeutic effects on adjuvant-induced rheumatoid model rats.The anti-rheumatic effects of the components of Epimedium are as follows:full composition >30 aXcohol segment >95 alcohol segment >water layer segment > polysaccharide segment.The mechanism of action may be related to improving the expression of inflammatory factors in the blood and inhibiting histopa

关 键 词：淫羊藿 拆分组分 佐剂型风湿模型 抗风湿作用 炎性因子 

分 类 号：R932[医药卫生—生药学]