肿瘤细胞上清液对小鼠糖尿病足溃疡愈合的影响  

Tumor cells supernatant promotes the healing of diabetic foot ulcers in mice through increasing the expression of VEGF-A, α-SMA and Vimentin

作　　者：张明[1] 黄君[1] 任锐[1] 肖衡[2] 杜成友[2] Zhang Ming;Huang Jun;Ren Rui;Xiao Heng;Du Chengyou(Department of General Surgery,the First People's Hospital of Yibin,Yibin 644000,China;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Chongqing Medical University, Chongqing 416000,China)

机构地区：[1]四川省宜宾市第一人民医院普外科,宜宾644000 [2]重庆医科大学附属第一医院肝胆外科,416000

出　　处：《中华内分泌外科杂志》2018年第6期464-468,共5页Chinese Journal of Endocrine Surgery

基　　金：宜宾市卫计委资助项目([2014]350号).

摘　　要：目的观察肿瘤细胞上清液对小鼠糖尿病足溃疡愈合的影响及对VEGF-A、α-SMA和Vimentin表达变化的影响。方法将45只雄性BALB/c小鼠随机分成正常对照组(A组)、肿瘤细胞上清液干预组(B组)、糖尿病对照组(C组),每组15只。B组和C组建立2型糖尿病足溃疡模型,造模成功后第1天起B组用肿瘤上清液干预,A组和C组各用同等体积的普通培养基处理。造模后第1、3、7天,观察各组溃疡愈合情况,比较各组创面感染率及死亡率,HE染色观察各组溃疡病理生长情况,免疫组织化学检测各组创面组织中VEGF-A、α-SMA及Vimentin的表达。ELISA试剂盒检测肿瘤上清液中相关蛋白的含量及其稳定性。结果A(66.7%)、B(80%)组的溃疡自愈率好于C(33.3%)组,创面感染率(A组=0,B组=7.1%)及死亡率(A组=0、B组=6.7%)低于C组(40.0%、33.3%),差异有统计学意义(P<0.05)。HE染色结果显示A、B组较C组愈合时间短,表皮覆盖较明显;免疫组化显示在A、B组的创面组织中VEGF-A、α-SMA及Vimentin的表达水平高于C组。ELISA结果显示肿瘤上清液中的TGF-β可较高水平的稳定表达。结论肿瘤上清液能有效提升小鼠糖尿病足模型的创面愈合能力,TGF-β,VEGF-A、α-SMA与Vimentin在这个过程中起到重要的作用。Objective To investigate the effect of tumor cells supernatant on treatment of diabetic foot ulcer in mice and on the expression of VEGF-A, α-SMA and Vimentin. Methods A total of 45 male BALB/c mice were randomly divided into three groups: normal control group (group A) , tumor cell supernatant treated group (group B) , and diabetic control group (group C) . Mouse models of type 2 diabetic foot ulcers were established in group B and group C. After the first day of modeling, group B were treated with tumor cells supernatant and the other two groups were injected with equal volume of medium. At the 1st, 3rd and 7th day following model established, mouse ulcer area was observed in each group. The ulcer infection rate and mortality of mice were compared between each group. The ulcer tissue of each group was HE-stained and the expression of VEGF-A, α-SMA and Vimentin in each group was detected by immunohistochemistry (IHC) . ELISA assay was used to detect the relative protein levels and stability in tumor cells supernatant. Results The healing degree in group A (66.7%) and group B (80.0%) was better than that in group C (33.3%) and the infection rate (group A=0, group B=7.1%) and mortality (group A=0, group B=6.7%) were significantly lower than those of group C (40.0%, 33.3%) , and the difference was statistically significant (P<0.05) . Compared with group C, HE staining showed that the healing time of group A and B was shorter than group C, and the epidermal coverage was more obvious. The expression levels of VEGF-A, α-SMA and Vimentin detected by IHC in group A and B were significantly higher than those in group C. ELISA results showed high-level and stable TGF-β expression in the tumor cells supernatant. Conclusion The tumor cells supernatant can effectively promote the healing of diabetic foot ulcers in mice and TGF-β, VEGF-A, α-SMA and Vimentin play a very important role in ulcers healing process.

关 键 词：肿瘤细胞 糖尿病足 小鼠 血管内皮生长因子 

分 类 号：R587.2[医药卫生—内分泌]