中空多孔硫化镍立方体电化学传感检测多巴胺  

Determination of dopamine via electrochemical sensor modified with hollow porous nickel sulfide nanocubes

作　　者：祝浪浪 黄志恒 马焕 戴大响 顾春川 刘红英 ZHU Lang-lang;HUANG Zhi-heng;MA Huan;DAI Da-xiang;GU Chun-chuan;LIU Hong-ying(College of Life Information Science &Instrument Engineering,Hangzhou Dianzi University, Hangzhou 310018;Deparment of Clinical Laboratory,Hangzhou Cancer Hospital,Hangzhou 310002)

机构地区：[1]杭州电子科技大学生命信息与仪器工程学院,杭州310018 [2]杭州市肿瘤医院检验科,杭州310002

出　　处：《分析试验室》2019年第1期60-64,共5页Chinese Journal of Analysis Laboratory

基　　金：杭州市社会发展科研项目(20160533B70);浙江省医药卫生计划(2017KY533);浙江省基础公益项目(LGF18H200005);2016年浙江省科协育才工程(2016YCGC007);国家自然科学基金(21405029)项目资助

摘　　要：以Ni-Co普鲁士蓝类似物为前驱体通过水热法在高温条件下利用碱性化学蚀刻和柯肯达尔效应的离子交换制备了中空多孔纳米硫化镍立方体,采用扫描透射电镜对材料进行表征。利用滴涂法制备基于中空多孔纳米硫化镍立方体修饰电极,用此电化学传感器建立了多巴胺定量检测方法。在最佳条件下,多巴胺溶液浓度在2～300μmol/L范围内与峰电流呈线性关系,r2=0. 9914,检出限为0. 68μmol/L。对尿液中多巴胺的含量进行了检测,样品加标回收率为99. 5%～103. 0%,RSD <5. 0%。方法可用于人体尿液、血清等样品中多巴胺的检测。In this paper,hollow porous nickel sulfide nanocubes(Ni S)were prepared using a solvothermal method in which Ni-Co prussian blue analogue(PBA)nanocubes were employed as a template.During the reaction,Ni-Co PBA nanocubes were gradually converted to nickel sulfide nanocubes with a hollow porous structure through ion exchange and chemical etching.Field emission scanning electron microscope was used to characterize the structure of Ni S nanocubes.Subsequently,Ni S nanocubes were used to build an electrochemical sensor for the determination of dopamine.Under the optimal condition,dopamine had a good linear relationship between peak current and its concentration within 2-300 μmol/L with r^2=0.9914.The limit of detection was 0.68 μmol/L.Furthermore,urine samples were determined by the electrochemical sensor,and the recoveries were99.5%-103.0% with a low relative standard deviation less than 5.0%.This method was applied for detecting dopamine in human urine and serum samples.

关 键 词：硫化镍 电化学传感 多巴胺 尿液 测定 

分 类 号：R446[医药卫生—诊断学]