鹦鹉热衣原体巨噬细胞感染增强因子对宿主细胞产生前炎症细胞因子和凋亡的影响  

Effect of microphage infectivity potentiator of Chlamydia psittaci on the production of proinflammatory cytokines and apoptosis in host cells

作　　者：曾心靛 张婉琪 符玺宗 周鹏[1] 周朴帆 唐婷 陈曦 胡春生[3] 陈丽丽[1] ZENG Xin--dian;ZHANG Wan-qi;FU Xi-zong;ZHOU Peng;ZHOU Pu-fan;TANG Ting;CHEN Xi;HU Chun-sheng;CHEN Li-li(School of Public Health,University of South China,Hengyang ,Hunan 421001,China;Chuanshaa College,University of South China,Hengyang,Hunan 421001,China;Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410005,China)

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001 [2]南华大学船山学院,湖南衡阳421001 [3]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《实用预防医学》2019年第2期129-133,共5页Practical Preventive Medicine

基　　金：国家自然科学基金项目(81572011;81001318);湖南省大学生研究性学习和创新性实验计划项目(1038)

摘　　要：目的研究鹦鹉热衣原体(Chlamydia psittaci,Cps)巨噬细胞感染增强因子(microphage infectivity potentiator,Mip)在诱导人单核细胞THP-1产生前炎症细胞因子和对HeLa细胞凋亡的作用。方法用不同浓度的Cps Mip重组蛋白作用THP-1细胞,ELISA检测前炎症细胞因子IL-8和TNF-α的表达量;用荧光染色法和流式细胞术分析重组Cps Mip对HeLa细胞凋亡的作用。结果重组Cps Mip能以时间、剂量依赖方式诱导THP-1细胞分泌前炎症细胞因子IL-8和TNF-α;不同Cps Mip蛋白浓度实验组刺激THP-1细胞产生的前炎症细胞因子IL-8和TNF-α水平显著高于PBS对照组(P<0. 05),当Cps Mip为16μg/ml时,所产生的前炎症细胞因子IL-8和TNF-α的量最高,分别为105. 01 pg/ml和50. 52 pg/ml。荧光染色法和流式细胞术检测细胞凋亡率,发现Cps Mip对星形孢菌素(staurosporine,STS)处理的HeLa细胞有一定的抑凋亡作用,在20μg/ml Cps Mip刺激后的细胞凋亡率比未刺激组下降了4. 48%(P<0. 01)。结论 Cps Mip蛋白能诱导THP-1细胞表达并分泌前炎症细胞因子IL-8和TNF-α; Cps Mip具有一定抑制HeLa细胞凋亡的作用。Objective To study the role of microphage infectivity potentiator(Mip) of Chlamydia psittaci(C. psittaci) on the production of proinflammatory cytokines in human acute monocytic leukemia cell line(THP-1) cells and apoptosis inhibition in HeLa cells. Methods Different concentration of recombinant Mip protein was used to stimulate THP-1 cells,and the expression of interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α) was detected by enzyme-linked immunosorbent assay(ELISA). The number of apoptosis cells was tested by fluorescence staining and flow cytometry after HeLa cells were stimulated with recombinant Mip protein. Results C. psittaci Mip could stimulate THP-1 cells to produce IL-8 and TNF-α in a dose-and time-dependent manner. The levels of IL-8 and TNF-α in THP-1 cells treated with different concentration of recombinant Mip protein were significantly higher than those in phosphate buffer saline treated groups(both P< 0.05). The production of IL-8(105.01 pg/ml) and TNF-α(50.52 pg/ml) reached the highest levels when the concentration of recombinant Mip protein was16 μg/ml. The fluorescent Hoechst 32258 staining and flow cytometric assay showed that C. psittaci Mip protein could inhibit the apoptosis of staurosporine-treated HeLa cells to some extent,and the rate of apoptosis cells reduced 4.48% when the concentration of recombinant Mip protein was 20 μg/ml. Conclusions C. psittaci Mip protein can stimulate THP-1 cells to produce IL-8and TNF-α and inhibit HeLa cell apoptosis.

关 键 词：鹦鹉热衣原体 CPS MIP IL-8 TNF-α 凋亡 

分 类 号：R374.2[医药卫生—病原生物学]