艾灸对新生小鼠脑卒中后抑郁模型色氨酸羟化酶2和单胺氧化酶A表达的影响  

Effect of Moxibustion on Expressions of Typtophan Hydroxylase-2 and Monoamine Oxidase A in Post-Stroke Depression Mice

作　　者：肖爱娇[1] 刘结民 龚丽丽[1] 欧阳昕[2] 陈明人 XIAO Aijiao;LIU Jiemin;GONG Lili;OUYANG Xin;CHEN Mingren(College of Basic Medical Science,Jiangxi University of Traditional Chinese Medicine,Nanehang 330004 ,Jiangxi,China;College of Moxibustion,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi,China)

机构地区：[1]江西中医药大学基础医学院,江西南昌330004 [2]江西中医药大学灸学院,江西南昌330004

出　　处：《辽宁中医杂志》2019年第1期178-180,I0008,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81660819);江西省科技厅重点研发项目(20161BBH80053);江西省卫生计生委中医药科研计划课题(2018A369);江西中医药大学课题(2014ZR018;2015JZZDXK024)

摘　　要：目的:观察艾灸对新生小鼠脑卒中后抑郁模型中缝核色氨酸羟化酶2(TPH2)以及大脑皮质单胺氧化酶A(MAO-A)蛋白表达的影响,研究艾灸治疗新生小鼠脑卒中后抑郁的作用机制。方法:7 d新生小鼠,随机分为假手术组、脑卒中后抑郁模型组和艾灸组。采用复合刺激制备新生小鼠脑卒中后抑郁模型,即于出生后7 d给予新生小鼠脑缺血缺氧刺激,并于术后3w始给予21d慢性不可预见性温和应激。采用荧光免疫组织化学法检测小鼠中缝核TPH2蛋白表达和大脑皮质MAO-A蛋白表达。结果:脑卒中后抑郁模型组小鼠中缝核TPH2阳性细胞数[(7.43±2.44)个]低于假手术组[(29.43±9.50)个],大脑皮质MAO-A阳性细胞数[(16.57±3.21)个]高于假手术组[(2.14±0.90)个];与模型组相比,艾灸组小鼠中缝核TPH2阳性细胞数[(23.29±8.71)个]增多,大脑皮质MAO-A阳性细胞数[(7.43±3.26)个]减少。结论:艾灸改善新生小鼠脑卒中后抑郁的效果,可能与其提高中缝核TPH2表达、降低大脑皮质MAO-A表达有关。Objective:The effects of moxibustion on the expressions of typtophan hydroxylase-2(TPH2) in median raphe nuclei and monoamine oxidase A(MAO-A) in the cortex were investigated in order to explore the mechanism of its antidepressant effect in post-stroke depression mice.Methods:Post-stroke depression model was induced by hypoxia and ischemia at 7 d postnatal in combination with chronic unexpected mild stress(CUMS) for 21 days since 3 weeks after surgery.Immunofluorescent staining was employed to detect the expression of TPH2 and MAO-A.Results:TPH2-positive cells(7.43±2.44)in median raphe nuclei decreased in model group compared to those in sham group(29.43±9.50),and MAO-A-positive cells(16.57±3.21)in cerebral cortex increased in model group compared to those in sham group(2.14±0.90),while TPH2-positive cells(23.29±8.71)in median raphe nuclei increased and MAO-A-positive cells(7.43±3.26)in cerebral cortex decreased in moxibustion-treated group compared to those in model group.Conclusion:Moxibustion improved the symptoms of post-stroke depression,which might be through regulating the expressions of TPH2 and MAO-A.

关 键 词：脑卒中后抑郁 慢性不可预见性温和应激 艾灸 色氨酸羟化酶2 单胺氧化酶A 

分 类 号：R245.81[医药卫生—针灸推拿学] R-332[医药卫生—中医临床基础]