扶正抗癌方通过逆转HepG2细胞EMT抗肝癌的实验研究  

Effect of Fuzheng Kang'ai Formula(扶正抗癌方) on Anti-hepatoma by Reversing EMT Transformation of HepG2 Cells

作　　者：方瑜 肖海娟 李仁廷 杨洋 陈光伟 FANG Yu;XIAO Hai-mei;LI Ren-ting;YANG Yang;CHEN Guang-wei(Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712046,China;Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712000,China)

机构地区：[1]陕西中医大学,陕西咸阳712046 [2]陕西中医药大学附属医院,陕西咸阳712000

出　　处：《中医药导报》2019年第1期60-63,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省中医药管理局项目(JCMS036)

摘　　要：目的:探讨扶正抗癌方对TGF-β1诱导的HepG2细胞EMT的影响及扶正抗癌方抑制肝癌侵袭与转移的机制。方法:建立肝癌HepG2细胞EMT模型并将其分为模型组、顺铂组、扶正抗癌方组、联合用药组,分别加入扶正抗癌方与顺铂含药血清,通过黏附实验、划痕实验、Transwell实验测定细胞的侵袭及转移能力,并检测EMT相关蛋白的表达。结果:各给药组细胞的黏附率、划痕的愈合率、转移的HepG2细胞数均低于模型组(P<0.05或P<0.01),联合用药组转移的HepG2细胞数低于顺铂组(P<0.01);各给药组HepG2细胞中Vimentin表达低于模型组,而E-cadherin表达高于模型组(P<0.05或P<0.01),联合用药组HepG2细胞中Vimentin表达低于顺铂组,而E-cadherin表达高于顺铂组(P<0.01)。结论:扶正抗癌方通过上调上皮标志蛋白E-cadherin的表达,下调间质细胞标记物Vimentin的表达,降低HepG2细胞运动和侵袭性,逆转其EMT进程。Objective: To explore the effect of Fuzheng Kang’ai Formula on TGF-β1-induced EMT of HepG2 cells and the mechanism of Fuzheng Kang’ai Formula on inhibiting the invasion and metastasis of hepatocellular carcinoma. Methods: Establised HepG2 cells EMT model and divided them into model group, Fuzheng Kang’ai Formula group, DDP group, Combination group, respectively joined Fuzheng Kang’ai Formula Recipe and Cisplatin-containing serum;We used cell-matrix adhesion assay, scratch test, transwell invasion and migration assay to determine the ability of HepG2 cells to adhere, invade and migrate;Western blot was used to detect the expression of EMT-related proteins. Results: The adhesion rate of the cells, healing rate of the scratches and transferred HepG2 cells in each medication group were lower than those in the model group(P<0.05 or P<0.01). The number of HepG2 cells transferred from Combination group was lower than that in the DDP group(P<0.01);The medication groups showed lower expression of Vimentin in HepG2 cells andhigher expression of E-cadherin than model group(P <0.05 or P <0.01). The expression of Vimentin was statistically significant in HepG2 cells. The combination group showed lower expression of Vimentin andhigher expression of E-cadherin than that of the DDP group(P<0.01). Conclusion: Fuzheng Kang’ai Formula can increase the expression of Ecadherin in the HepG2 cells, down-regulate the expression of the stromal cell marker Vimentin, reduce the single cell movement and invasiveness, and reverse the EMT process.

关 键 词：扶正抗癌方 DDP HEPG2 迁移与侵袭 EMT E-cadherin VIMENTIN 

分 类 号：R273[医药卫生—中西医结合]