葛根素对丙烯酰胺染毒大鼠中枢神经能量代谢影响  

Effect of puerarin on energy metabolism of central nervous system in acrylamide-exposed rats

作　　者：关亚丽 李鹏飞[1] 程月发[1] 闫立成 金玲[1] 王妍[1] 彭晨 GUAN Yali;LI Pengfei;CHENG Yuefa;YAN Lieheng;JIN Ling;WANG Yan;PENG Chen(Jitang College of North China University of Science and Technology,Tangshan,Hebei 063210,China)

机构地区：[1]华北理工大学冀唐学院,河北唐山063210 [2]华北理工大学公共卫生学院,063210

出　　处：《中国职业医学》2018年第6期740-743,共4页China Occupational Medicine

基　　金：河北省高等学校科学技术研究青年基金(QN201722)

摘　　要：目的观察葛根素对丙烯酰胺染毒大鼠中枢神经能量代谢的影响。方法无特定病原体级成年雄性SD大鼠随机分为对照组、模型组和葛根素低、中、高剂量组,每组10只。模型组和葛根素低、中、高剂量组大鼠均予剂量为30 mg/kg体质量丙烯酰胺腹腔注射染毒,对照组大鼠腹腔注射等体积的0. 9%氯化钠溶液;染毒1 h后,3个葛根素剂量组大鼠分别予剂量为40、80和160 mg/kg体质量葛根素腹腔注射,每周3次,连续给药4周。于第4周,断头处死大鼠后分离其脑和脊髓,检测脑和脊髓线粒体中腺苷二磷酸(ADP)/腺苷三磷酸(ATP)比值、ATP合成酶活力及线粒体膜电位。结果与对照组比较,模型组和3个葛根素剂量组大鼠脑与脊髓组织线粒体中ADP/ATP比值均升高(P <0. 05),ATP合成酶活力和线粒体膜电位均下降(P <0. 05)。与模型组比较,3个葛根素剂量组大鼠脑与脊髓组织线粒体中ADP/ATP比值均下降(P <0. 05),脑组织线粒体中线粒体膜电位均升高(P <0. 05)。与模型组比较,葛根素高剂量组大鼠脑与脊髓组织ATP合成酶活力均升高(P <0. 05),葛根素中、高剂量组大鼠脊髓组织线粒体膜电位均升高(P <0. 05)。随着葛根素剂量的增加,大鼠脑和脊髓组织线粒体中ADP/ATP比值下降(P <0. 05),线粒体膜电位升高(P <0. 05)。结论葛根素可能通过调节线粒体ATP合成酶增强大鼠中枢神经线粒体的能量代谢功能,并对丙烯酰胺染毒大鼠中枢神经线粒体膜有一定的保护作用。Objective To observe the effect of puerarin on energy metabolism of the central nervous in acrylamide-exposed rats. Methods Specific pathogen free adult male SD rats were randomly divided into control group,model group,and puerarin low-,medium-,and high-dose groups,with 10 rats in each group. Intraperitoneal injection of acrylamide was given to rats in model group,and puerarin low-,medium-,and high-dose groups( 30 mg/kg body weight). Rats in control group were intraperitoneally injected with equal volume of 0. 9% sodium chloride solution. Rats in the puerarin low-,medium-,and high-dose groups were given puerarin of 40,80 and 160 mg/kg body weight,respectively after one hour of acrylamide exposure,three times a week for continuous 4 weeks. In the 4th week,the rats were sacrificed,the brain and spinal cord were isolated,and the ratio of adenosine diphosphate( ADP)/adenosine triphosphate( ATP),ATP activity and mitochondrial membrane potential( MMP) in brain and spinal cord tissue mitochondria were detected. Results The ADP/ATP ratio increased in mitochondria of brain and spinal cord tissue in model group and the three puerarin treatment groups( P < 0. 05),meanwhile the ATP synthase activity and MMP decreased compared with control group( P < 0. 05). The ADP/ATP ratio decreased in mitochondria of brain and spinal cord tissue( P < 0. 05),while MMP increased in mitochondria of brain tissue in the three puerarin treatment groups compared with model group( P < 0. 05). The ATP synthase activity increased in mitochondria of brain and spinal cord tissue of puerarin high-dose group( P < 0. 05),and MMP increased in mitochondria of spinal cord tissue of puerarin medium-does group and puerarin high-does group when compared with the model group( P < 0. 05). The ADP/ATP ratio in mitochondria of rat brain and spinal cord decreased with the increase of puerarin doses( P < 0. 05). The MMP of rat brain and spinal cord increased with the increase of puerarin dose( P < 0. 05). Conclusion Puerarin enhances the energy metabolism function

关 键 词：葛根素 丙烯酰胺 脑 脊髓 线粒体 腺苷三磷酸 膜电位 大鼠 

分 类 号：R135.1[医药卫生—劳动卫生]