大血藤药材三氯甲烷部位HPLC指纹图谱研究  

HPLC Fingerprint of Chloroform Extracts of Sargentodoxae Caulis

作　　者：韦汉燕[1] 韦佳林 王晓华[1] 戴平[1] WEI Hanyan;WEI Jialin;WANG Xiaohua;DAI Ping(Guilin Medical University,Guiling 541004,China;Fapon Biotech Inc.Dongguan 523808,China)

机构地区：[1]桂林医学院,广西桂林541004 [2]菲鹏生物股份有限公司,广东东莞523808

出　　处：《中国民族民间医药》2019年第1期31-35,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：2017年度广西高等教育本科教学改革工程项目:基于QbD理念及PBL模式的HPLC药物分析实验教学改革与实践(编号:2017JGA261)

摘　　要：目的:应用Rp-HPLC (DAD)对大血藤药材三氯甲烷部位进行指纹图谱研究。方法:Diamonsil spursilC18分析柱,乙腈-水梯度洗脱,流速0. 8 m L/min,检测波长270 nm。柱温:30℃;进样量为10μL。结果:建立了大血藤药材三氯甲烷部位指纹图谱,确认19个共有峰,指认了3个峰,指纹图谱的相似度达到0. 801以上,所建立的大血藤药材三氯甲烷部位指纹图谱测定方法精密度、重复性和稳定性良好。结论:建立了专属性强的大血藤药材三氯甲烷部位指纹图谱,可为大血藤质量控制提供依据。Objective A fingerprint of chloroform extracts of Sargentodoxae Caulis has been studied and provides the quality information.Method Diamonsil spursil C18 column was used,with mixtures of acetonitrile and water as mobile phase in a gradient mode. The wavelength of measurement was 270 nm.Column temperature was 30℃;Inject volume was 10μL.Result The HPLC characteristic fingerprint of chloroform extracts of Sargentodoxae Caulis with 19 commons peaks,and three of them were identified by comparing with reference subslances.The values of similarity was higher than 0.801.The HPLC method showed better results of stability,precision,and repeatability.Conclusion The fingerprint of chloroform extracts of Sargentodoxae Caulis has been establish.It may be practical value for the quality control of sample for Sargentodoxae Caulis.

关 键 词：大血藤 HPLC 指纹图谱 

分 类 号：R927.2[医药卫生—药学]