HAART治疗后不同免疫应答人群T细胞特征研究  

Immune characteristics of T cells in different immune responses cohorts after receiving HAART

作　　者：雷洁 李金程[1] 丁海波[1] 唐甜[1] 张子宁[1] 韩晓旭[1] 姜拥军[1] LEI Jie;LI Jincheng;DING Haibo;TANG Tian;ZHANG Zining;HAN Xiaoxu;JIANG Yongjun(Key Laboratory of AIDS Immunology of National Health and Family Planning Commission,Key Laboratory of AIDS Immunology of Liaoning Province,Key Laboratory of AIDS Immunology of Chinese Academy of Medical Sciences, Department of Laboratory Medicine,First Affiliated Hospital of China Medical University,Shengyang 110001,China)

机构地区：[1]中国医科大学附属第一医院检验科国家卫生健康委员会艾滋病免疫学重点实验室辽宁省艾滋病免疫学重点实验室中国医学科学院艾滋病免疫学重点实验室

出　　处：《免疫学杂志》2019年第2期143-150,共8页Immunological Journal

基　　金：国家科技重大专项(2012ZX10001-006)

摘　　要：目的探讨HIV感染者经高效抗逆转录病毒治疗(HAART)后不同免疫应答情况下T细胞的免疫特征。方法选取中国医科大学附属第一医院经HAART治疗的HIV感染者27例,按照免疫应答情况将其分为免疫无应答者(INR,14例)和免疫应答者(RES,13例),另有健康对照者(NC)25例。利用流式细胞仪对上述人群CD4^+T细胞数量、胸腺输出功能、活化及凋亡情况进行分析比较。结果 HIV感染者在HAART治疗后CD4^+T细胞数量持续增长,RES组CD4^+T细胞数量明显高于INR组。感染者治疗后的胸腺输出功能与CD4^+T细胞数量呈正相关(r=0.503 7,P=0.012 1),INR的胸腺输出功能显著低于RES(P<0.05)。感染者CD4^+T细胞活化水平与CD4^+T细胞数量呈负相关(r=-0.469 7,P=0.017 8),INR的活化水平显著高于RES(P<0.01)。感染者细胞凋亡水平与CD4^+T细胞数量呈反比(r=-0.547 2,P=0.018 7),INR的CD4^+T细胞凋亡水平明显高于RES(P<0.01)。结论较低的胸腺输出功能、过度的活化及凋亡水平对抗病毒治疗后的免疫重建都将产生不利的影响。This study was performed to investigate T cell immune characteristics of immune responders and immune nonresponders who have received HAART. Total of 27 HIV-infected individuals from the First Affiliated Hospital of China Medical University were selected, who were divided into immune responders(RES) and immune non-responders(INR) according to their immune responses, and another 25 healthy controls(NC) were also included in the study. CD4^+T cell counts, thymic output function, immune activation and cell apoptosis of these individuals were analyzed by flow cytometry. Data showed that the CD4^+T cell counts in HIV-infected individuals continued to increase after HAART treatment, and it was significantly higher in RES than that in INR. The thymic output function of HIV-infected individuals was positively correlated with CD4^+T cell counts(r=0.5037, P=0.012 1), and its level was significantly lower in INR than that in RES(P<0.05). The immune activation level of HIV-infected individuals was negatively correlated with CD4^+T cell count(r=-0.469 7, P=0.017 8), and INR showed even much higher level of immune activation than those of RES(P<0.01). The level of CD4^+T cell apoptosis in INR was significantly higher when compared with RES(P<0.01) and it was negativelycorrelated with CD4^+T cell count(r=-0.547 2,P=0.018 7). In conclusion, impaired thymic output function, overactivation and apoptosis might exertnegative impact on immune reconstruction after receiving HAART.

关 键 词：HIV HAART治疗 CD4^+T细胞 免疫应答者 

分 类 号：R392.12[医药卫生—免疫学]