重度脂标本对ALT及ELISA检测的影响及处理  

The effects on detection of ALT and ELISA of blood specimens with severe lipemia and specimens' treatment

作　　者：黄秀琳[1] 阳禄平 陈敏 汤耕耘 王芳[1] HUANG Xiulin;YANG Luping;CHEN Min;TANG Gengyun;WANG Fang(Chongqing Blood Center,Chongqing 400015,China)

机构地区：[1]重庆市血液中心,重庆400015

出　　处：《中国输血杂志》2018年第11期1298-1300,共3页Chinese Journal of Blood Transfusion

摘　　要：目的研究本中心无偿献血重度脂标本对ALT及ELISA检测结果的影响,为建立ALT重度脂血标本处理的标准操作规程提供理论依据。方法 100例常规检测中因重度脂血ALT无检测结果的标本,取上层脂浆后原管标本再次检测ALT,吸出的脂浆以10 956. 4 g高速离心10 min;分离下层清液再次检测ALT和HBs Ag,抗-HCV,抗-HIV+P24、抗-TP。结果 100例标本经吸出上层脂浆的简单处理后,有39例标本ALT可以检测出结果,而经10956. 4 g高速离心10 min后ALT全部能检出,且仅有5例检测值>50 U/L,吸出上层脂浆和高速离心处理后检测结果95%的分布区间分别为(26. 9±20. 6)和(27. 2±21. 2),2种处理方法的结果经t检验无显著性差异;高速离心处理前后ELISA检测定性结果完全一致。结论 4种ELISA试剂在重度脂血标本检测中未出现HBs Ag、抗-HCV、抗-HIV+P24、抗-TP反应性标本漏检情况,将高速离心用于ALT重度脂血标本的处理能够解决常规离心无法检测ALT的问题,从而极大地降低血液报废率。Objective To explore the impact of blood specimens with severe lipemia on donation and to provide theoretical basis for establishing SOP for the treatment of ALT severe lipemia specimens. Methods 100 severe lipemia specimens were enrolled for ALT tests. After the removal of the top fat slurry,the specimen was again tested for ALT. The removed fat slurry went through a 10 min centrifugation at10 956. 4 g,Separating the lower supernatant for yet another ALT test. HBs Ag,anti-HCV,anti-HIV+P24,anti-TP were also tested. Results ALT could be detected by in 39 of the 100 original tube specimens with fat removed;However,ALT could be detected in all 100 samples after centrifugation. 5 cases yielded readings were over 50 U/L. The qualitative ELISA results were identical before and after the centrifugation. Conclusion The four ELISA reagents in the study did not appear to be missing the reactive results of HBs Ag,anti-HCV,anti-HIV+ P24 or anti-TP in the detection of severe lipemia specimens. The high speed centrifugation approach is capable of enhancing ATL detection and thereby greatly reducing the rate of blood discarded.

关 键 词：重度脂血 标本处理 

分 类 号：R446.11[医药卫生—诊断学]