丹参注射液对重症急性胰腺炎大鼠心脏的保护作用  

The Protective Effects of Danshen Injection on the Myocardium of Rats with Severe Acute Pancreatitis

作　　者：李培武[1] 杨莉[2] 丁卓奇[2] 岳金芳[2] 杨伟钦 米红 向长勤 刘凤斌[1] LI Peiwu;YANG Li;DING Zhuoqi;YUE Jinfang;YANG Weiqin;MI Hong;XIANG Changqin;LIU Fengbin(Spleen and Stomach Department, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China;Gastroenterology Department, First People's Hospital of Kashgar, Kashgar 844099 Xinjiang, China;Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong,China)

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]新疆喀什地区第一人民医院,新疆喀什844099 [3]广州中医药大学,广东广州510405

出　　处：《中药新药与临床药理》2019年第2期184-188,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(2014A020209071);广州中医药大学第一附属医院创新科研团队项目(2015LP01);广东省第二批名中医师承项目(粤中医办函[2015]93号)

摘　　要：目的探讨丹参注射液对重症急性胰腺炎(SAP)大鼠心肌损伤的保护作用及机制。方法选取雄性SD大鼠60只,随机分为6组,即假手术组,模型组,乌司他丁组(2×104U·kg^(-1)),丹参注射液高、中、低剂量组(15、7.5、3.75 g·kg^(-1))。实验采用逆行胆胰管灌注5%牛磺胆酸钠溶液复制SAP大鼠模型,造模后3 h,按组别尾静脉注射生理盐水、乌司他丁、不同剂量丹参注射液。6 h后苏木素-伊红(HE)染色法观察各组大鼠胰腺及心肌的病理变化,并检测大鼠心肌细胞Na+-K+-ATP酶活性,qPCR法检测心肌组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)mRNA表达量。结果与假手术组比较,模型组的大鼠胰腺、心肌损伤严重,病理评分明显升高(P<0.05),心肌Na+-K+-ATP酶活性明显降低(P<0.05),TNF-α、IL-1βmRNA的表达量明显升高(P<0.05);与模型组比较,各给药组的胰腺和心肌组织病理评分明显降低(P<0.05),丹参注射液高、中剂量组的Na+-K+-ATP酶活性明显升高(P<0.05),丹参注射液高剂量组的TNF-α、IL-1βmRNA表达量明显降低(P<0.05)。结论丹参注射液可能通过提高心肌细胞Na+-K+-ATP酶活性,减少心肌组织中TNF-α、IL-1β的表达,对心肌起保护作用。Objective To investigate the curative effect and mechanism of Danshen injection on severe acute pancreatitis-related myocardial injury. Methods Sixty male SD rats were randomly divided into 6 groups: Sham group,model group,ulinastatin group and Danshen injection (high dose 15 g·kg^-1,midium dose 7.5 g·kg^-1,low dose 3.75 g·kg^-1) . The rats in model group,ulinastatin group and Danshen injection groups were reconstructed via retrograde cholangiopancreatic injection of 5% sodium taurocholate. After 3 h, normal saline, ulinastatin and Danshen injection were injected by the tail vein correspondingly. After 6 h,the pathological changes of pancreas and myocardium were observed,and the Na^+-K^+-ATPase activity,and the mRNA expression of TNF-α,IL-1β and IL- 6 in rat cardiomyocytes were detected. Results Compared with the Sham group,the pancreatic,myocardial injury and pathological scores of the model group were significantly increased (P < 0.05),the activity of Na^+-K^+-ATPase was significantly decreased (P < 0.05), the mRNA expression levels of TNF- α and IL- 1β were significantly increased (P < 0.05). Compared with the model group, the pancreatic and cardiac pathological scores of the ulinastatin group and Danshen injection groups were significantly decreased (P < 0.05). Compared with the model group,the activity of Na^+-K^+-ATPase in the myocardium of Danshen injection medium and high dose groups were significantly increased (P < 0.05),and the mRNA expression of TNF-α and IL-1β of Danshen injection high dose group were significantly decreased (P < 0.05). Conclusion Danshen injection may significantly improve the activity of Na^+-K^+-ATPase in myocardial cells and decrease the expression of TNF-α and IL-1β in mRNA in myocardium and protect the myocardium.

关 键 词：丹参注射液 重症急性胰腺炎 NA^+-K^+-ATP酶 肿瘤坏死因子-α 白细胞介素-1Β 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]