养阴舒肝颗粒对肝郁证模型小鼠行为学及脑内单胺类神经递质的影响  

Effects of Yangyin Shugan Granule on Praxeology and Monoamine Neurotransmitters in Mice with Liver Depression Syndrome

作　　者：王吉菊 刘建[2] 黄旭春[2] 曾玉燕[2] 曹晓静[2] 王小云[2] WANG Jiju;LIU Jian;HUANG Xuchun;ZENG Yuyan;CAO Xiaojing;WANG Xiaoyun(.The Second Clinical College,Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;Guangdong Hospital of TCM,Guangzhou 510120 Guangdong,China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510405 [2]广东省中医院,广东广州510120

出　　处：《中药新药与临床药理》2019年第1期47-51,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金项目(2017A030313728;2018A030310508);广东省中医药管理局科研项目(20181101);国家中医药管理局王小云全国名老中医经验传承工作室建设项目;广东省中医院岭南妇科流派工作室建设项目

摘　　要：目的探讨养阴舒肝颗粒对肝郁证模型小鼠行为学和脑内单胺类神经递质的影响。方法采用孤养和慢性不可预见性温和刺激法制备肝郁证模型,观察小鼠造模期间的一般情况、体质量变化,取造模成功小鼠72只,按照体质量随机分为模型组,养阴舒肝颗粒低、中、高剂量组(3、6、12 g·kg^(-1)),逍遥丸组及盐酸氟西汀组,另取12只正常小鼠作为对照组,连续灌胃28 d。采用悬尾实验观察各组小鼠悬尾不动时间;测定额叶皮质和海马的质量;以ELISA试剂盒测定皮质和海马内多巴胺(DA)、5-羟色胺(5-HT)的含量。结果与正常对照组比较,模型组小鼠悬尾不动时间明显延长,额叶皮质和海马内DA、5-HT含量均明显下降,差异有统计学意义(P<0.05,P<0.01)。与模型组比较,养阴舒肝颗粒高剂量组的小鼠悬尾不动时间明显缩短(P<0.05),小鼠额叶皮质DA含量明显升高(P<0.05);养阴舒肝颗粒中剂量组、高剂量组小鼠额叶皮质5-HT含量以及海马区DA、5-HT含量明显升高(P<0.05)。结论养阴舒肝颗粒可以有效改善模型小鼠的肝郁状态,其机制可能与调节肝郁证小鼠的神经递质,升高额叶皮质及海马内的DA、5-HT有关。Objective To study the effects of Yangyin Shugan(YYSG) granule on praxeology and monoamineneurotransmitters in mice with liver depression syndrome. Methods The models of liver depression syndrome wereinduced by isolation and chronic unpredictable mild stimulation. Seventy-two ICR mice with liver depressionsyndrome were randomly divided into 6 groups:model group,YYSG low,medium and high dose groups(3,6,12 g·kg^-1),Xiaoyao pill group and fluoxetine group,another 12 mice were taken as the normal control group. After28 days of continuous gavage, the time of tail suspension, the weights of frontal cortex and hippocampus, thecontents of dopamine(DA) and 5-hydroxytryptamine(5-HT) in the cortex and hippocampus were observed inmice of every group. Results Compared with the blank control group,the time of tail suspension was significantlyprolonged,and the contents of DA and 5-HT in the frontal cortex and hippocampus decreased significantly in themodel group(P < 0.01,P < 0.05). Compared with the model group,the time of tail suspension in YYSG granulehigh dose group was significantly shorter, and the contents of DA in the frontal cortex and hippocampus weresignificantly higher(P < 0.05);the contents of 5-HT in the frontal cortex,and the contents of 5-HT and DA inthe hippocampus of medium and high dose YYSG groups were significantly higher(P < 0.05). Conclusion YYSG granule could effectively improve the state of liver depression,and its mechanism may be related to the regulation ofneurotransmitters,elevation the contents of DA and 5-HT in the frontal cortex and hippocampus.

关 键 词：养阴舒肝颗粒 肝郁证 行为学 单胺类神经递质 多巴胺 5-羟色胺 

分 类 号：R285.5[医药卫生—中药学]