SEB对人HaCaT细胞株S100A8和S100A9表达的影响  

Effects of SEB on S100A8 and S100A9 Expression in HaCaT Cells

作　　者：崔晶[1] 金珊[1] 金承龙[1] 方宇辉[1] 朱莲花[1] 元星花 金哲虎[1] CUI Jing;JIN Shan;JIN Chenglong;FANG Yuhui;ZHU Lianhua;YUAN Xinghua;JIN Zhehu(Department of Dermatology,Yanbian University Hospital,Yanji 133000,China)

机构地区：[1]延边大学附属医院皮肤科,吉林延吉133000

出　　处：《中国皮肤性病学杂志》2019年第2期149-153,共5页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(81560503)

摘　　要：目的金黄色葡萄球菌肠毒素B(Staphylococcus aureus enterotoxin B,SEB)刺激HaCaT细胞后观察S100A8、S100A9、白介素-1α(IL-1ɑ)和白介素-17 (IL-17)表达的影响。进一步阐明机体组织损伤相关分子模式(damage associated molecularpatterns,DAMP)与先天免疫对AD发病的影响。方法采用MTT法检测SEB对HaCaT细胞的细胞毒性。通过蛋白免疫印迹法测定细胞内外DAMP分子S100A8、S100A9蛋白表达。用酶联免疫吸附实验检测HaCaT细胞培养液中的IL-1α、IL-17的表达情况。结果在24、48、72 h,不同浓度SEB对HaCaT细胞无毒性作用。SEB刺激组细胞内外S100A8、S100A9蛋白表达较对照组增多。在不同浓度的SEB处理下IL-1α表达水平呈上升趋势。100 ng/mL SEB刺激组较对照组相比,IL-17表达水平明显升高。结论 SEB可能通过上调DAMP分子S100A8、S100A9蛋白以及IL-1α、IL-17炎症因子的表达,参与AD的发病机制。Objective To detect the S100A8,S100A9,IL-1a and IL-17 expression in the Staphylococcus aureus enterotoxin B(SEB)stimulated HaCaT cells,and clarify the impact of damage associated molecular patterns(DAMPs)and innate immunity on the pathogenesis of AD.Methods MTT assay was used to determine the cytotoxicity of SEB on the HaCaT cells.The expression of DAMP molecules S100A8 and S100A9 proteins were detected by Western blot.The expression of IL-1α and IL-17were measured by enzyme linked immunosorbent assay(ELISA).Results Different concentrations of SEB had no cytotoxicity on HaCaT cells at 24,48 and 72 h.The expression of intracellular and extracellular S100A8 and S100A9 were significantly increased in the SEB-treat group.The expression of IL-1α was increased in the SEB-treat HaCaT cells in concentration dependent manner.The expression of IL-17 was significantly increased in the 100 ng/mL SEB-treat group.Conclusion SEB participates the pathogenesis of AD through upregulating S100A8,S100A9,IL-1α and IL-17.

关 键 词：特应性皮炎 金黄色葡萄球菌肠毒素B S100A8 S100A9 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]