泡型肝包虫患者外周血树突状细胞成熟度  

Maturation of dendritic cells in peripheral blood of patients with alveolar echinococcosis

作　　者：马晓静[1] 尚梅[1] 尹启超[1] 马婕[1] 熊华[1] 罗伟[1] 耿惠[1] 樊海宁[1] 王海久[1] 崔森[1] 李占全[1] 冀林华[1] MA Xiaojing;SHANG Mei;YIN Qichao;MA Jie;XIONG Hua;LUO Wei;GEN Hui;FAN Haining;WANG Haijiu;CUI Sen;LI Zhanquan;JI Linhua(Department of Hematology,Affiliated Hospital of Qinghai University,Xining,Qinghai 810001,China)

机构地区：[1]青海大学附属医院血液科,青海西宁810001

出　　处：《中国热带医学》2019年第2期111-115,共5页China Tropical Medicine

基　　金：青海大学附属医院中青年科研基金重点项目(No.ASRF-2015-YB-03)

摘　　要：目的通过研究泡型肝包虫病(alveolar echinococcosis, AE)患者外周血中树突状细胞(dendritic cells, DC)的成熟度,探究DC在AE患者免疫发病机制中的作用。方法选取2015年9月—2016年6月于青海大学附属医院初诊并经病理证实为AE患者及健康体检者各20例,流式细胞仪检测外周血CD80+细胞、CD86+细胞、CD83+细胞及(CD80%+CD83%+CD86%)/CD14%比值。选取2016年6月—2017年2月于该院初诊并经病理证实为AE患者及健康体检者各30例,利用流式细胞仪检测DC表面CD83、CD86、CD80表达率。结果 AE组与对照组外周血检测DC表面的共刺激分子(CD80%+CD83%+CD86%)/CD14%差异无统计学意义(P>0.05),CD86细胞表达(7.94±1.32)较对照组低(P<0.05)。AE组外周血单核源性培养DC表面的共刺激分子CD80、CD83、CD86的表达水平(41.71±8.16、39.69±8.05、50.79±7.72)均低于对照组(62.27±5.94、56.37±7.33、72.00±7.36)(P均<0.05)。结论 AE患者外周血DC成熟度比健康对照组低,存在成熟障碍,可能会导致宿主抗原提呈功能下降,机体抗感染能力降低,从而促进了多房棘球蚴感染的进展。Objective To study the maturation of dendritic cells in peripheral blood of patients with alveolarechinococcosis in Qinghai, and to explore the role of dendritic cells in the disease. Methods A total of 20 cases newlydiagnosed and confirmed by pathology of AE patients and 20 cases of healthy subjects were selected in Qinghai UniversityHospital from September 2015 to June 2016, peripheral CD80^+ cells, CD86^+ cells, CD83^+ cells and the ratio of(CD80%^+CD83%+CD86%)/CD14% were detected by flow cytometry. And 30 cases of newly diagnosed and confirmed by pathology ofAE patients and 30 cases of healthy subjects were selected in the hospital from September 2015 to June 2016. The expressionof CD83, CD86 and CD80 on DC surface was detected by flow cytometry. Results(CD80%^+CD83%^+CD86%)/CD14% werenot statistically in healthy controls and AE group(P>0.05). The percentage of CD86(7.94±1.32)in patients with AE waslower than those in healthy controls(P<0.05). The percentage of CD80+, CD83+, CD86^+ in patients with AE(41.71±8.16,39.69±8.05, 50.79±7.72)were significantly lower than those in healthy controls(62.27±5.94, 56.37±7.33, 72.00±7.36)(P <0.05). Conclusions DC in patients with AE have higher maturity expression than DC in healthy volunteers. There is amature barrier, leading to AE patients with decreased antigen presentation function, lack of immune response and the bodyanti-infective ability promote the progress of multi-cell echinococcosis infection.

关 键 词：泡型肝包虫病 树突状细胞 单核细胞 成熟度 

分 类 号：R18[医药卫生—流行病学] R532.32[医药卫生—公共卫生与预防医学]