岭南特色饮片制佛手的质量标准研究  

Quality Standard of Lingnan Characteristic Sliced Herbal Medicine of Processed Fructus Citri Sarcodactylis

作　　者：张秋霞[1] 汪金玉[1] 陈燕霞[2] 陈康[1] 王红阳 黄玉梅 朱银姬 高明[1] ZHANG Qiu-Xia;WANG Jin-Yu;CHEN Yan-Xia;CHEN Kang;WANG Hong-Yang;HUANG Yu-Mei;ZHU Yin-Ji;GAO Ming(College of Chinese Herbal Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;The First Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;Sliced Chinese Medicine Factory,Guangzhou Medica Materia Company,Guangzhou510360 Guangdong,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学第一附属医院,广东广州510405 [3]广州市药材公司中药饮片厂,广东广州510360

出　　处：《广州中医药大学学报》2019年第2期256-262,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省教育厅2016年重点平台及科研项目--青年创新人才项目(编号:2016KQNCX022);广州中医药大学2017年省级大学生创新训练计划项目(编号:201710572066)

摘　　要：【目的】为制佛手饮片质量标准的制定提供参考依据。【方法】采用薄层色谱法对不同批次制佛手进行鉴别研究,采用高效液相色谱法对其橙皮苷、5,7-二甲氧基香豆素和5-羟甲基糠醛进行含量测定。【结果】在环己烷—乙酸乙酯(1.5∶1)的展开系统,以5,7-二甲氧基香豆素和5-羟甲基糠醛为对照品,14批制佛手饮品的薄层色谱在相同位置显示斑点一致;橙皮苷含量在0.142～0.788 mg/g之间;5,7-二甲氧基香豆素含量在0.642～1.747 mg/g之间;5-羟甲基糠醛含量0.028～0.901 mg/g之间。【结论】本研究所建立的薄层鉴别方法和高效液相色谱含量测定方法可应用于岭南特色饮片制佛手的质量控制及标准制定。Objective To provide a reference for the quality standard of sliced herbal medicine of processed Fructus Citri Sarcodactylis.with Lingnan(the south of the five ridges)characteristic Methods Different patches of Fructus Citri Sarcodactylis were identified by thin layer chromatography,and the contents of hesperidin,5,7-dimethoxycoumarin,and 5-hydroxymethylfurfural were assayed by high performance liquid chromatography(HPLC).Results With 5,7-dimethoxycoumarin and 5-hydroxymethylfurfuralthe as the references,the results of thin layer chromatography for 14 different patches of Fructus Citri Sarcodactylis in the developing system of ethyl cyclohexylideneacetate(1.5∶1)showed that similar spots were present at the same sites.The content of hesperidin was within 0.1418-0.788 mg/g,5,7-dimethoxycoumarin was within 0.642-1.747 mg/g,and 5-hydroxymethylfurfural was within 0.028-0.901 mg/g.Conclusion Thin layer chromatography and HPLC established in the study can be applied for the quality control and standard setting of sliced herbal medicine of processed Fructus Citri Sarcodactylis with Lingnan characteristics.

关 键 词：制佛手 薄层色谱 高效液相色谱 橙皮苷 5 7-二甲氧基香豆素 5-羟甲基糠醛 

分 类 号：R284.1[医药卫生—中药学]