一种新型优化的经口气管插管法对大鼠心肌梗死模型的建立  

Establishment of rat myocardial infarction model by a new optimized orotracheal intubation method

作　　者：赵新军[1] 李荣[1] 吴伟[1] 卿立金[1] 罗川晋[1] 褚庆民[1] 李靖[1] 都治伊 邱艺俊[1] ZHAO Xin-jun;LI Rong;WU Wei;QING Li-jin;LUO Chuan-jin;CHU Qing-min;LI Jing;DU Zhi-yi;QIU Yi-jun(Department of Cardiology,the first Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一附属医院心血管科,广东省广州市510405

出　　处：《中国心血管病研究》2019年第1期87-91,共5页Chinese Journal of Cardiovascular Research

基　　金：2017广东省中医药局科研项目(20171086);2017年广东省教育厅重点平台及科研项目(自然科学)(2017KTSCX047);2017广州中医药大学第一附属医院青年科研人才培优项目(2017QN09).

摘　　要：目的评估一种新型优化的经口气管插管法是否提高大鼠心肌梗死模型远期存活率。方法45只Wistar雄性大鼠,随机分为三组,每组15只,另设对照组10只,分别采用新型优化经口气管插管法、传统气管切开法和无辅助呼吸组建立人工气道,结扎冠状动脉左前降支制作心肌梗死模型,从心肌梗死大鼠存活率、造模手术时间及从术后4周大鼠心脏整体形态、心脏结构和心功能变化等方面分析,判断新型优化的经口气管插管法对大鼠心肌梗死模型存活率的影响。结果4周后,新型优化经口气管插管组大鼠平均存活时间为(25.07±7.75)d,与无辅助呼吸组(11.20±12.84)d和气管切开组(20.47±11.19)d对比明显延长(P<O.01,P<0.05),经口优化气管插管组的存活率最高,存活率达86.67%,而无辅助呼吸组和气管切开组分别为33.33%和66.67%;和正常对照组比较,心肌梗死大鼠心脏体积明显增大,左心室腔扩大,室壁变薄,部分室壁瘤形成;超声心动图检测显示,新型优化经口气管插管法、传统气管切开法和无辅助呼吸组三组心肌梗死造模成功大鼠的LVEF和LVFS均明显降低,其中主要参数LVEF分别为(38.84±5.99)%、(38.53±7.23)%、(37.18±12.38)%,和正常对照组(70.67±7.97)%对比,差异具有统计学意义;LVEDD和LVEDS均明显增大,其中LVEDD分别为(8.88±0.95)mm、(8.98±1.33)mm和(9.77±1.05)mm,三组数据对比差异未见统计学意义。结论新型优化经口气管插管法的应用能够为大鼠心肌梗死模型的制作迅速备好人工气道,可以明显缩短手术时间,提高心肌梗死动物模型的远期存活率,具有显著的优越性。Objective To evaluate a new optimized way to improve the success rate of rat myocardial infarction model by oral intubation.Methods 45 male Wistar rats were randomly divided into three groups,15 rats in each group and 10 as normal control group.The artificial airway was established by the new optimized tracheal intubation method,the traditional tracheotomy method and the non-assisted breathing method repectively;and the myocardial infarction model was established by ligating the left anterior descending branch of coronary artery.The survival rate,the mean operation time,the observation of cardiac gross morphology and the changes of cardiac structure and heart function of surviving myocardial infarction rats were analyzed.Results 4 weeks after the operation,the mean survival time of the rats in the oral optimization tracheal intubation group (25.07±7.75)days was significantly longer than that in the non-assisted breathing group (11.20±12.84)days and tracheotomy group (20.47±11.19)days (P<0.01,P<0.05).The survival rate of myocardial infarction rats in the three groups was evaluated.The survival rate in the oral optimization tracheal intubation group reached 86.67%, however,the survival rate in the non-assisted breathing group and tracheotomy group were 33.33%and 66.67%. Compared with the normal control group,the heart volume of myocardial infarction rats were significantly increased,left ventricular cavity enlarged,ventricular wall thinned,and ventricular aneurysms formed.LVEF and LVFS in the three groups were significantly decreased (P<0.001),the main parameter (LVEF)were (38.84±5.99)%,(38.53±7.23)%,(37.18±12.38)%,compared with the normal control group (70.67±7.97)%,the difference is statistically significant.The LVEDD and LVEDS were significantly increased (P<0.001).The main parameter (LVEDD)were (8.88±0.95)ram,(8.98±1.33)mm and (9.77±1.05)ram.There was no significant difference among the three groups.Conclusion The application of oral optimization tracheal intubation method can quickly prepare artifici

关 键 词：新型优化经口气管插管法 心肌梗死模型 大鼠 

分 类 号：Q95-33[生物学—动物学] R542.22[医药卫生—心血管疾病]