Vero细胞培养H3N2流感病毒冷适应株条件的优化  

Optimization of condition for culture of cold-adapted influenza H3N2 virus strain in Vero cells

作　　者：纪燕燕 范家佑[1] JI Yan-yan;FAN Jia-you(College of Pharmacy,Guizhou University,Guiyang 550025,Guizhou Province,China)

机构地区：[1]贵州大学药学院,贵州贵阳550025

出　　处：《中国生物制品学杂志》2019年第1期7-9,共3页Chinese Journal of Biologicals

基　　金：云南省创新团队(2015HC027);中国医学科学院医学科技创新团队(2016-12M-3-026)

摘　　要：目的对Vero细胞培养H3N2流感病毒条件进行优化。方法使用Vero细胞培养H3N2流感病毒,对病毒感染复数(MOI)、吸附时间、维持液中NaHCO_3含量、TPCK-胰酶终浓度和细胞病变效应(CPE)等培养条件进行优化。结果在MOI为0. 05,病毒吸附1. 5 h,病毒维持液中6. 6%NaHCO_3溶液加入量为2%,TPCK-胰酶终浓度为1 mg/L,40%～50%细胞出现CPE情况下收获病毒,血凝(hemagglutination,HA)效价最高。结论优化后的培养条件可用于大规模流感疫苗的生产。Objective To optimize the condition for culture of influenza H3N2 virus in Vero cells.Methods Influenza H3N2 virus was cultured in Vero cells,and the culture condition including MOI,adsorption time,sodium hydrogen carbonate concentration in maintenance medium,final concentration of TPCK-trypsin and CPE were optimized.Results The hemagglutination(HA)titer of influenza H3N2 virus reached the maximum when the MOI was 0.05,the adsorption time was 1.5 h,the 6.6% sodium hydrogen carbonate concentration in maintenance medium was 2%,final concentration of TPCK-trypsin was 1 mg/L,and 40% ~ 50% CPE appeared.Conclusion The optimized condition was suitable for large-scale production of influenza vaccine.

关 键 词：流感病毒 H3N2 VERO细胞 培养条件 

分 类 号：R373.13[医药卫生—病原生物学]