重组人干扰素α1b含片对大鼠免疫功能的调节作用  

Regulatory effect of recombinant human interferon α1b lozenges on immune function of rats

作　　者：何利思 蒋伟[1] 韦康[2] 田建明[2] 王妍[1] HE Li-si;JIANG Wei;WEI Kang;TIAN Jian-ming;WANG Yan(School of Applied Chemistry and Biology,Shenzhen Polytechnic,Shenzhen 518055,Guangdong Province,China)

机构地区：[1]深圳职业技术学院应用化学与生物技术学院,广东深圳518055 [2]吉林省中医药科学院,吉林长春130012

出　　处：《中国生物制品学杂志》2019年第1期49-53,共5页Chinese Journal of Biologicals

基　　金：深圳市科技创新委2017自由探索项目(JCYJ20170306144523128)

摘　　要：目的探讨重组人干扰素α1b(recombinant human interferonα1b,rhIFNα1b)含片对大鼠免疫功能的调节作用。方法将大鼠按体重随机分为模型组(空白基质片干粉)、阳性对照组(匹多莫德)及rhIFNα1b含片低(1 000 IU/片)、中(10 000 IU/片)、高(50 000 IU/片)剂量组,每组14只,给予相应药物,每日1次,连续给药3周,于首次给药第7、9天经腹腔注射环磷酰胺,60 mg/kg,同时设正常对照组(10只),末次给药8 h后开始禁食,24 h后称量大鼠体重,计算胸腺及脾脏系数,检测血清及脾脏细胞中白细胞介素-2(interleukin-2,IL-2)、IL-4、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IFNα水平,同时检测血清、唾液中IgG及IgA水平。结果与正常对照组比较,模型组大鼠体重及胸腺系数均明显降低(P <0. 05),血清中IL-2、IL-4、TNF-α、IFNα、IgG及IgA水平均明显下降(P <0. 01),脾脏中IL-4、TNF-α及IFNα水平均明显下降(P <0. 01),唾液中IgG及IgA水平均明显下降(P <0.01)。与模型组比较,各剂量rhIFNα1b含片组大鼠的体重、胸腺及脾脏系数差异均无统计学意义(P> 0. 05);血清及脾脏中IL-2、IL-4、TNF-α及IFNα水平有不同程度的升高;血液及唾液中IgA水平明显升高(P <0. 05)。结论中、高剂量的rhIFNα1b含片对大鼠免疫功能具有明显的改善作用。Objective To investigate the regulatory effect of recombinant human interferon α1b(rhIFNα1b)lozenges on immune function of rats.Methods Rats were divided into five groups by weight,14 for each.The rats in model,positive control and three test groups were treated with empty matrix powder,pidotimod and rIFNα1b at dosages of 1 000,10 000 and 50 000 IU/lozenge respectively,once a day for 3 weeks,and injected i.p.with cyclophosphamide at a dosage of 60 mg/kg on days 7 and 9,using ten normal rats as control.The rats were fasted 8 h and weighed 24 h after the last administration,of which the thymus and spleen coefficients were calculated,and the IL-2,IL-4,tumor necrosis factor-α(TNF-α) and IFNα levels in sera and spenocytes as well as IgG and IgA levels in sera and saliva were determined.Results As compared with those of normal control,the bodyweight and thymus coefficient(P< 0.05),the IL-2,IL-4,TNF-α,IFNα,IgG and IgA levels in sera(each P< 0.01),as well as IL-4,TNF-α and IFNα levels in spleen(P< 0.01)and IgG and IgA levels in saliva(P< 0.01)of rats in model group decreased significantly.However,as compared with those in model group,the bodyweight and thymus and spleen coefficients of rats treated with rhIFNα1b lozenges at various dosages showed no significant difference(P >0.05),while the IL-2,IL-4,TNF-α and IFNα levels in sera and spleen increased at different degrees,and the IgA levels in sera and saliva increased significantly(P< 0.05).Conclusion The IFNα1b lozenges at moderate and high dosages improved the immune function of rats significantly.

关 键 词：重组人干扰素Α1B 含片 免疫功能 环磷酰胺 白细胞介素 肿瘤坏死因子 

分 类 号：R373.23[医药卫生—病原生物学] Q75[医药卫生—基础医学]