α2-巨球蛋白的分离纯化及临床应用  

Separation,purification and clinical application of α2-macroglobulin

作　　者：皇甫超济 党明安 赵雄 章金刚 HUANGFU Chao-ji;DANG Ming-an;ZHAO Xiong;ZHANG Jin-gang(Institute of Health Service and Transfusion Medicine,Academy of Military Medical Sciences,Beijing 100850,China)

机构地区：[1]军事医学研究院卫生勤务与血液研究所,北京100850 [2]西宁联勤保障中心兰州疾病预防控制中心,甘肃兰州730020 [3]河南省食品药品审评查验中心,河南郑州450018

出　　处：《中国生物制品学杂志》2019年第1期106-109,共4页Chinese Journal of Biologicals

基　　金：国家863计划资助项目(2012AA021902);国家自然科学基金青年基金(81803171)

摘　　要：α2-巨球蛋白(α2-macroglobulin,α2-M)是一种大分子糖蛋白,主要存在于人体的血浆中,是一种广谱蛋白酶抑制剂,可清除内源性及外源性蛋白酶。制备α2-M工艺的起始原料多为人血浆,实验室制备工艺大多结合使用免疫亲和层析、凝胶过滤、Cibacron蓝色琼脂糖亲和层析等,而规模化制备工艺一般先使用硫酸铵、利凡诺、聚乙二醇等方法进行粗分离,再使用锌离子亲和层析、凝胶过滤等方法进行精细分离。α2-M用于治疗放射性皮肤黏膜溃疡损伤、放射性肺炎、放射性膀胱炎、角膜炎及角膜溃疡具有良好效果。α2-M还可作为判断肝纤维化分期、胰腺炎病情轻重的一个生物标记物。本文主要就α2-M的制备工艺及临床应用作一综述。α2-Macroglobulin(α2-M)is a large molecular glycoprotein which mainly exists in human plasma.As a broad spectrum inhibitor of proteases,α2-M has the ability of removing both endogenous and exogenesis proteases.The original material for preparation of α2-M is mainly human plasma,while the laboratory preparation procedure mainly includes immuno-affinity chromatography,gel filtration and Cibacron blue agarose affinity chromatography.However,the pilot preparation procedure usually consists of crude separation with ammonium sulfate,rivanol or polyethylene glycol and further purification by zinc ion affinity chromatography or gel filtration.α2-M presents good curative effect on radiation induced ulcer of skin and mucosa,radiological pneumonia,radiological cystitis,keratitis and corneal ulcer.Meanwhile,α2-M may be used as a biomarker for judgment of stage of liver fibrosis and the severity of pancreatitis.This paper reviews the preparation procedure and clinical application of α2-M.

关 键 词：Α2巨球蛋白 分离纯化 临床应用 

分 类 号：R446.11[医药卫生—诊断学]