科尔沁沙地小叶锦鸡儿群落土壤氨氧化细菌多样性  

Diversity of soil ammonia-oxidizing bacteria under Caragana microphylla community in Horqin Sandy Land

作　　者：高婷婷 张颖 杨盛 崔振波 曹成有 GAO Ting-ting;ZHANG ying;YA-NG Sheng;CUI Zhen-bo;CAO Cheng-you(College of Life and Health Sciences,Northeastern University,Shenyang 110169, China)

机构地区：[1]东北大学生命科学与健康学院,沈阳110169

出　　处：《生态学杂志》2019年第1期113-120,共8页Chinese Journal of Ecology

基　　金：国家重点研发计划项目(2016YFC0500803);国家自然科学基金项目(41371505)资助

摘　　要：土壤氮素形态的转换主要由相关微生物驱动完成,其中由氨氧化细菌(AOB)主导的氨氧化过程是硝化作用的限速步骤。在土地沙漠化正逆过程中,植被和土壤条件的变化也会对土壤微生物群落产生显著影响。本研究在科尔沁沙地典型地区选择小叶锦鸡儿天然群落、30年生小叶锦鸡儿人工固沙群落和流动沙丘为对象,以amoA基因为标记,采用克隆文库和Q-PCR技术,研究了沙地小叶锦鸡儿群落土壤氨氧化细菌多样性和菌群结构及其变化特征。结果表明:小叶锦鸡儿群落土壤氨氧化细菌多样性较低,均为亚硝化螺菌属(Nitrosospira);在土地沙化正逆过程中,土壤氨氧化菌群结构的变化主要体现在数量上,而基本组分变化不大;流动沙丘上重建小叶锦鸡儿植被可恢复土壤氨氧化菌群结构;土壤全氮和全磷是影响AOB群落结构的主要环境因子。Soil nitrogen transformation is primarily driven by related functional soil microbes.Ammonia oxidation by ammonia-oxidizing bacteria( AOB) is the rate-limiting step of nitrification.During the processes of desertification and restoration,soil microbial communities are significantly affected by the changes of vegetation and soil. In this study,we investigated the diversity and community structure of soil AOB marked by amoA gene with clone library and quantitative PCR techniques under 30-year-old Caragana microphylla sand-fixation plantation and moving sandy dune in Horqin Sandy Land. We found that the diversity of AOB in the C. microphylla soil was very low,with only some Nitrosospira species being observed. During the positive and negative processes of desertification,the structure of soil AOB communities mainly varied in quantity,while the composition of soil AOB communities remained unchanged. These results indicate that soil AOB community structure in moving sandy dune can be restored to its original condition by planting C. microphylla. Soil total N and P concentrations are major environmental factors affecting AOB community structure.

关 键 词：小叶锦鸡儿 流动沙丘 氨氧化细菌 多样性 AMOA基因 

分 类 号：S714.3[农业科学—林学]