不同处理下油菜RbohA、RbohD基因的表达特性分析  

Expression analysis of RbohA and RbohD genes in Brassica campestris under different treatments

作　　者：张腾国[1] 赖晶 李萍[1] 孙万仓[2] 刁志宏[1] 王娟[1] 郑晟[1] ZHANG Teng-guo;LAI Jing;LI Ping;SUN Wan-cang;DIAO Zhi-hong;WANG Juan;ZHENG Sheng(School of Life Sciences,Northwest Normal University,Lanzhou 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃农业大学农学院,兰州730070

出　　处：《生态学杂志》2019年第1期173-180,共8页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31460099)资助

摘　　要：以白菜型冬油菜"陇油6号"和"天油2号"为供试材料,利用实时荧光定量PCR技术研究RbohA、RbohD基因在不同组织及不同处理下的相对表达。结果表明:RbohA、RbohD基因在这两个冬油菜品种的根、茎、叶以及下胚轴中均有表达,没有组织特异性;低温(2℃)、盐(200 mmol·L-1)及PEG-6000模拟干旱胁迫可以诱导RbohA、RbohD基因的表达,且"陇油6号"油菜中RbohA、RbohD基因的表达水平高于"天油2号"油菜;外源ABA和H2O2处理油菜幼苗后,RbohA、RbohD基因的表达均有不同程度升高;用DPI(NADPH氧化酶抑制剂)、U0126(MPKK专一性抑制剂)及DMTU(H2O2清除剂)预处理后再分别进行低温或盐胁迫,与单独低温或盐胁迫结果相比,"陇油6号"和"天油2号"油菜中RbohA、RbohD基因的表达量明显降低;油菜RbohA、RbohD基因在响应低温、盐、PEG-6000模拟干旱胁迫过程中发挥作用; RbohA、RbohD基因的表达受ABA和H2O2的诱导,H2O2和MAP激酶参与了低温及盐胁迫对RbohA、RbohD基因表达的诱导过程。The expression of RbohA and RbohD genes in different tissues of Brassica campestris L.cultivars "Longyou 6"and "Tianyou 2"seedlings under different treatments was detected by real-time fluorescence quantitative PCR. The results showed that RbohA and RbohD genes were expressed in roots,stems,leaves,and hypocotyls of both cultivars without tissue specificity. The expression of RbohA and RbohD was induced by different treatments,including low temperature( 2 ℃),salt stress( 200 mmol·L-1),and PEG-6000 simulated drought stress. The expression of both genes was higher in"Longyou 6"than in"Tianyou 2". The expression of RbohA and RbohD genes increased with various degrees in seedlings under exogenous ABA and H2O2 treatments.Compared to the treatment of low temperature or salt stress alone,the expression of RbohA and RbohD was significantly decreased in both cultivars when they were pretreated with DPI( NADPH oxidase inhibitor),U0126( MPKK specific inhibitor) and DMTU( H2O2scavenger) with low temperature or salt stress. Such a result indicated that RbohA and RbohD are important for responses to low temperature,salt,and PEG-6000 simulated drought stress. ABA and H2O2 induced the expression of RbohA and RbohD. H2O2 and MAP kinase were involved in the induction of RbohA and RbohD gene expression under low temperature and salt stress.

关 键 词：油菜 RbohA RbohD 组织特异性 基因表达 

分 类 号：S565.4[农业科学—作物学]