麦芪降糖丸对2型糖尿病ob/ob小鼠糖脂水平的影响  

Effect of Maiqi-Jiangtang pill on the glycolipid level in type 2 diabetic ob/ob mice

作　　者：方芳[1] 田小叶 沙娜·吾肯 唐晓丽 吴金英[1] 谯志文 陈晓雪 陈小红 孙建宁[1] Fang Fang;Tian Xiaoye;Sha Na?Wuken;Tang Xiaoli;Wu Jinying;Qiao Zhiwen;Chen Xiaoxue;Chen Xiaohong;Sun Jianning(Department of Pharrnaeology of Chinese Materia Medica,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Chongqing Si Ke Institute of Materia Medica Co.Ltd.,Chongqing 400039,China)

机构地区：[1]北京中医药大学中药学院中药药理系,102488 [2]重庆思科药物研究所有限公司,400039

出　　处：《国际中医中药杂志》2019年第1期39-43,共5页International Journal of Traditional Chinese Medicine

基　　金：北京市自然科学基金(7144222).

摘　　要：目的研究麦芪降糖丸对2型糖尿病ob/ob小鼠糖脂水平的影响。方法将ob/ob小鼠按随机数字表法分为模型组及麦芪降糖丸高、中、低剂量组,另设C57BL/6J小鼠为正常对照组,每组13只。麦芪降糖丸组高、中、低剂量组灌胃麦芪降糖丸溶液8、4、2 g/kg,正常对照组和模型组小鼠灌胃等体积0.5%羧甲基纤维素钠,1次/d,连续10周。每周监测小鼠体重;测定各组小鼠空腹血糖;采用生化法测定小鼠血清TG、TC、HDL-C、LDL-C水平和肝脏TG、TC水平;计算肝脏指数。结果各组小鼠体重无明显差异。与模型组比较,麦芪降糖丸中、低剂量组小鼠空腹血糖[(7.43±1.71)mmol/L、(7.84±1.09)mmol/L比(8.95±0.96)mmol/L]降低(P<0.05),高剂量组小鼠血清TG[(0.93±0.16)mmol/L比(1.18±0.26)mmol/L]、LDL-C[(2.10±0.51)mmol/L比(2.56±0.44)mmol/L]水平降低(P<0.05),肝脏重量[(3.52±0.26)g比(3.98±0.35)g]与肝脏指数[(0.063±0.004)比(0.071±0.006)]降低(P<0.05,P<0.01),血清HDL-C/LDL-C比值[(2.40±0.39)比(1.96±0.24)]增高(P<0.01);麦芪降糖丸低剂量组肝脏TG[(0.63±0.25)mmol/g比(1.05±0.67)mmol/g]水平降低,血清HDL-C/LDL-C比值[(2.30±0.44)比(1.96±0.24)]升高(P<0.05)。结论麦芪降糖丸可降低2型糖尿病ob/ob小鼠糖脂水平。Objective To investigate the effects of Maiqi-Jiangtang pill on the glycolipid level in type 2 diabetic ob/ob mice.Methods The 8-week old male ob/ob mice were randomly divided into Maiqi-Jiangtang pill high- (8 g/kg),medium- (4 g/kg),low- (2 g/kg) dose groups. All the mice orally adiministered with the drugs once a day for 10 weeks. The same week age normal C57BL/6J control mice and ob/ob model group mice were orally administered with the equal volume solvent. The body weight per week were recorded. The fasting blood-glucose (FBG) was measured by glycemic instrument. The content of TG,TC,HDL-C,LDL-C in serum,and TG and TC content in liver were determined by biochemical method. The liver index was calculated.Results Compared with ob/ob model group,there was no significant change in body weight of mice administered with Maiqi-Jiangtang pill for 10 weeks. Compared with the model group,the low-,medium- dose Maiqi-Jiangtang pill could significantly decrease the FBG (7.43 ± 1.71 mmol/L,7.84 ± 1.09 mmol/L vs. 8.95 ± 0.96mmol/L),the high- dose Maiqi-Jiangtang pill could significantly reduce the TG (0.93 ± 0.16 mmol/L vs. 1.18 ± 0.26 mmol/L) and LDL-C (2.10 ± 0.51 mmol/L vs. 2.56 ± 0.44 mmol/L) content in serum of ob/ob mice (P<0.05),increase the HDL-C/LDL-C ratio (2.40 ± 0.39 vs. 1.96 ± 0.24) in serum (P<0.01),decrease the liver weight (3.52 ± 0.26 g vs. 3.98 ± 0.35 g) and the liver index (0.063 ± 0.004 vs. 0.071 ± 0.006) (P<0.05). Compared with the model group,the low dose Maiqi-Jiangtang pill could also significantly decrease the TG level (0.63 ± 0.25 mmol/g vs. 1.05 ± 0.67 mmol/g) in liver and significantly increase the HDL-C/LDL-C ratio (2.30 ± 0.44 vs. 1.96 ± 0.24) in serum (P<0.05).Conclusions The Maiqi-Jiangtang pill can reduce lipid in serum and liver of ob/ob mice while it can decrease the blood glucose,which need to further study its mechanism.

关 键 词：糖尿病 2型 麦芪降糖丸 胆固醇 降血脂药(中药) 降血糖药(中药) OB/OB小鼠 

分 类 号：R285.5[医药卫生—中药学]