乌头碱抗佐剂关节炎的药效学研究  

Study on pharmacodynamics of aconitine anti-adjuvant arthritis

作　　者：杜成成 沈建英 任汝静[2] 尹婷 洪坤 孙建辉[2] 罗俊 谭余庆[2] Du Chengcheng;Shen Jianying;Ren Rujing;Yin Ting;Hong Kun;Sun Jianhui;Luo Jun;Tan Yuqing(Department of Pharmacology,School of Basic Medical Sciences,Guizhou Medical University,Guizhou 550025,China;Institute of Chinese Materia Medical,China Academy of Chinese Medical Sciences,Beijing 100029,China)

机构地区：[1]贵州医科大学基础医学院,550025 [2]中国中医科学院中药研究所中药安全评价中心,北京100029

出　　处：《国际中医中药杂志》2019年第1期44-48,共5页International Journal of Traditional Chinese Medicine

基　　金：人力资源和社会保障部留学人员科技活动项目[人社厅函(2016)176号].

摘　　要：目的研究乌头碱对佐剂性关节炎模型大鼠的治疗作用。方法取Wistar大鼠50只,按随机数字表法分为空白组、模型组、甲氨蝶呤组、乌头碱低剂量组及乌头碱高剂量组。除空白组外,各组大鼠右后足皮内注射弗式完全佐剂致大鼠佐剂性关节炎模型。造模后第10天,乌头碱低、高剂量组分别灌胃25、100 μg/kg乌头碱,1次/d,甲氨蝶呤组灌胃0.5 mg/kg甲氨蝶呤,2次/周,连续给药19 d。末次给药后,使用足趾容积测量仪测量大鼠足肿胀度,按5级评分法计算大鼠关节炎指数,计算脾脏、胸腺指数,采用HE染色观察大鼠右足踝关节部位病理学改变。结果与模型组比较,造模后20、24、28 d,乌头碱低、高剂量组大鼠足跖肿胀度[20 d:(668.7±144.5)μl、(566.9±179.3)μl比(912.1±200.5)μl;24 d:(833.1±144.0)μl、(803.9±213.4)μl比(1 069.5±164.6)μl;28 d:(736.4±115.0)μl、(835.7±170.1)μl比(1 107.2±215.8)μl]降低(P<0.05或P<0.01);造模后18、24、28 d,乌头碱低、高剂量组大鼠关节炎指数评分[18 d:(3.1±0.7)分、(3.2±0.4)分比(3.8±0.6)分;24 d:(3.1±0.5)分、(3.4±0.5)分比(3.9±0.3)分;28 d:(2.7±0.6)分、(3.2±0.9)分比(4.0±0.0)分]降低(P<0.05);与模型组比较,乌头碱低、高剂量组及甲氨蝶呤组脾脏指数[(3.5±0.4)、(3.3±0.4)、(4.0±0.6)比(4.9±0.5)]降低(P<0.01)。结论乌头碱对佐剂性关节炎大鼠有一定的治疗作用。Objective To study the pharmacological function of the aconitine in treating adjuvant arthritis(AA).Methods Fifty rats were randomly divided into the control group,AA model group,methotrexate group(0.5 mg/kg),and aconitine groups of different dosages (25,100 μg/kg). Except the control group,each group was injection of intradermal Freund’s complete adjuvant (0.1 ml) into right hindpaw of rats to establish adjuvant arthritis model. On the 10th day after model onset,the aconitine were administered by gavage with 25,100 μg/kg once daily,and the methotrexate group was administered with 0.5 mg/kg methotrexate twice per week,and all groups were treated for 19 days. After the last administration,the foot swelling was measured by toe volume meter,arthritis index was calculated by 5-grade scoring method,spleen and thymus index were calculated,and the pathological changes of right ankle joint were observed by HE staining.Results After the model establishment,compared with the model group,the degree of swelling of the ankle at 20 days (668.7 ± 144.5 μl,566.9 ± 179.3 μl vs. 912.1 ± 200.5 μl),24 days (833.1 ± 144.0 μl,803.9 ± 213.4 μl vs. 1 069.5 ± 164.6 μl) and 28 days (736.4 ± 115.0 μl,835.7 ± 170.1 μl vs. 1 107.2 ± 215.8 μl) in the aconitine groups significantly decreased (P<0.05 or P<0.01). After the model establishment,compared with the model group,arthritic index scores at 18 days (3.1 ± 0.7,3.2 ± 0.4 vs. 3.8 ± 0.6),24 days (3.1 ± 0.5,3.4 ± 0.5 vs. 3.9 ± 0.3),28 days (2.7 ± 0.6,3.2 ± 0.9 vs. 4.0 ± 0.0) in the aconitine groups significantly decreased (P<0.05). Compared with the model group,the spleen index (3.5 ± 0.4,3.3 ± 0.4,4.0 ± 0.6 vs. 4.9 ± 0.5) respectively in the low and high dose group of aconitine and methotrexate group (P<0.01).Conclusion Aconitine has a certain degree therapeutic effect on AA rats.

关 键 词：乌头碱 关节炎 实验性 Freund佐剂 中药药效学 大鼠 

分 类 号：R285.5[医药卫生—中药学]