藤黄酸对人膀胱癌细胞BIU-87增殖及侵袭的影响  

Effects of garcinia acid on proliferation and invasion of human bladder cancer cell line

作　　者：谭大清 刘孝华 梁骏 吴建华 毛卫林 Tan Daqing;Liu Xiaohua;Liang Jun;Wu Jianhua;Mao Weilin(Department of Urology,University Hospital of Hubei University for Nationalities,Enshi 445000,China)

机构地区：[1]湖北民族学院附属民大医院泌尿外科,恩施445000

出　　处：《国际中医中药杂志》2019年第1期53-56,共4页International Journal of Traditional Chinese Medicine

摘　　要：目的观察藤黄酸对人膀胱癌细胞株BIU-87增殖、侵袭的影响,探讨其作用机制。方法体外培养BIU-87细胞,将细胞按随机数字表法分为空白对照组及藤黄酸低、中、高剂量组,藤黄酸低、中、高剂量组加入含20、40、80 μmol/L藤黄酸的新鲜培养基培养,空白对照组以普通培养基培养。干预24、48、72 h后,采用CCK8试剂盒检测各组细胞增殖抑制率;采用底部涂Matrigel胶的Transwell小室模型对BIU-87细胞进行体外侵袭研究;采用Western blot法检测BIU-87细胞内VEGF表达。结果与空白对照组比较,干预24、48、72 h后,藤黄酸低、中、高剂量组细胞增殖抑制率升高(P<0.05),且各组细胞增殖抑制率随时间延长而升高(P<0.05);藤黄酸低、中、高剂量组穿过滤膜的细胞数[(26±4)个、(41±4)个、(53±5)个比(119±7)个]降低(P<0.05),VEGF[(41.2±6.2)、(23.8±5.2)、(17.9±4.7)比(14.8±4.2)]表达水平降低。结论藤黄酸对人膀胱癌细胞的增殖和侵袭能力有明显抑制作用,其机制可能与下调VEGF表达有关。Objective To investigate the impacts of garcinia acid on the proliferation and invasion abilities of human bladder cancer cell line (BIU-87),and to study the possible molecular mechanisms.Methods The BIU-87 cells were cultured in vitro,and then the cells were divided into control group,low-dose,middle-dose,and high-dose garcinia acid groups. The cells in the drug groups were treated with 20,40,80 μmol/L of garcinia acid for 24,48,and 72 h,and control group were incubated by normal medium. The inhibition of proliferation of BIU-87 cells was performed using CCK8 assay. The abilities of BIU-87 cell invasion were assessed using Transwell chambers,and the expression levels of vascular endothelial growth factor (VEGF) were analyzed using Western Blot technology.Results Compared with control group,the proliferation inhibitory rates of cells after treatment with low-,middle-,and high-dose garcinia acid for 24,48,and 72 h were significantly decreased (P<0.05). Moreover,the proliferation inhibitory rates in different drug groups were greatly increased with the time extension. Compared with control group,the number of cells passing through the membrane(26 ± 4,41 ± 4,53 ± 5 vs. 119 ± 7) in low-,middle-,and high-dose garcinia acid group significantly decreased (P<0.05),the expression levels of VEGF (41.2 ± 6.2,23.8 ± 5.2,17.9 ± 4.7 vs. 14.8 ± 4.2) in low-,middle-,and high-dose garcinia acid group significantly decreased (P<0.05).Conclusions The Garcinia acid can inhibit the proliferation and invasion of BIU-87 cells via the down-regulation of VEGF expression.

关 键 词：细胞增殖 肿瘤侵润 血管内皮生长因子 藤黄酸 

分 类 号：R285[医药卫生—中药学]