姜厚朴饮片标准汤剂研究  

Research on standard decoction of ginger juice Magnoliae Officinalis Cortex

作　　者：荆文光 邓哲 孙晓波[1] 兰青山[2] 刘安 JING Wen-guang;DENG Zhe;SUN Xiao-bo;LAN Qing-shan;LIU An(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China;Traditional Chinese Medicine Institute of China National Traditional Chinese Medicine Corporation,Beijing 102600,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]中国中药有限公司科技研发部,北京102600 [3]中国中医科学院中药研究所,北京100700

出　　处：《中草药》2019年第1期83-89,共7页Chinese Traditional and Herbal Drugs

基　　金：国家中药标准化项目(ZYBZH-Y-ZY-45)

摘　　要：目的制备16批姜厚朴饮片标准汤剂,测定其出膏率、指标成分含量、转移率及指纹图谱等数据,为其配方颗粒的制备和质量评价提供参考。方法依照中药饮片标准汤剂制备要求,制备16批姜厚朴饮片标准汤剂,参照《中国药典》2015年版方法测定厚朴酚与和厚朴酚含量,计算两者总和的转移率,测定汤剂的出膏率及pH值,并绘制HPLC指纹图谱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱:0～15 min,12%～16%乙腈;15～30 min,16%～28%乙腈;30～42 min,28%～74%乙腈;42～55 min,74%～80%乙腈;柱温40℃;体积流量1 mL/min;检测波长294 nm。结果 16批姜厚朴饮片标准汤剂中厚朴酚与和厚朴酚总和的转移率为6.5%～12.0%,出膏率3.41%～7.14%,pH值4.63～5.43,并用中药色谱指纹图谱相似度评价系统(2012A)软件进行指纹图谱分析,找到了7个共有峰,指认4个,分别为木兰苷B(1号峰)、木兰苷A(2号峰)、和厚朴酚(6号峰)、厚朴酚(7号峰)。对16批姜厚朴饮片标准汤剂进行相似度评价,其相似度均大于0.69。同时,建立了姜厚朴饮片标准汤剂指纹图谱。结论所建立的汤剂制备方法稳定可行,分析方法精密度、稳定性和重复性良好,可用于姜厚朴饮片标准汤剂的质量评价。Objective To study the dry extract rate, determination and transfer rate of maker compounds, and fingerprint of standard decoction of ginger juice Magnoliae Officinalis Cortex(GJMOC) and provide a reference for the preparation and quality assessment of its dispensing granules by establishing 16 batches of standard decoction of GJMOC. Methods A total of 16 batches of GJMOC standard decoctions were prepared following literature requirements. The quantitative analysis method of magnolol and honokiol was according to Chinese Pharmacopoeia(2015 edition). The transfer rate of total magnolol and honokiol and extraction rate were calculated. the pH value was determined and HPLC fingerprint was established under a flow rate of 1 mL/min and eluted with a mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) in a gradient mode(0-15 min, 12%-16% A;15-30 min, 16%-28% A;30-42 min, 28%-74% A;42-55 min, 74%-80% A). The column temperature was set at 40℃ and the detection wavelength was 294 nm. Results By measuring the of 16 batches of standard decoction, the transfer rate of the sum of magnolol and honokiol ranged from 6.5% to 12.0%, the extraction rate was at a range of 3.41% to 7.14% and pH value was 4.63 to 5.43. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 A) was used to analyze and compare the fingerprints, and seven common peaks were determined and four were identified including magnoloside B(peak 1), magnoloside A(peak 2), honokiol(peak 6), and magnolol(peak 7). The similarity among 16 batches of standard decoction of GJMOC was evaluated, and the similarity was all greater than 0.69. Moreover, this study established an HPLC fingerprint analysis method of GJMOC standard decoction. Conclusion The preparation method established in this study is stable and feasible, and the analysis method shows good precision, stability, and repeatability in fingerprint analysis and it is suitable for evaluating the quality of standard decoction of GJMOC.

关 键 词：姜厚朴 标准汤剂 质量标准 配方颗粒 木兰苷B 木兰苷A 和厚朴酚:厚朴酚 指纹图谱 质量评价 

分 类 号：R286.02[医药卫生—中药学]