基于灰色关联度与正交偏最小二乘法分析的苍术挥发油燥性谱效关系研究  

Spectrum-effect relationship of dryness of Atractylodis Rhizoma volatile oil based on grey relational grade and orthogonal partial least squares analysis

作　　者：瞿领航 曹国胜 涂济源 石坤 王洁 徐依依 刘艳菊 QU Ling-hang;CAO Guo-sheng;TU Ji-yuan;SHI Kun;WANG Jie;XU Yi-yi;LIU Yan-ju(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Chinese Materia Medica Processing Engineering Center of Hubei Province,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430065 [2]湖北省中药炮制技术工程研究中心,湖北武汉430065

出　　处：《中草药》2019年第1期150-156,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81073051);国家自然科学基金资助项目(81541080);国家科技基础性专项资助项目(2014FY111100-2)

摘　　要：目的研究苍术挥发油的气相色谱-质谱(GC-MS)联用指纹图谱与苍术燥性效应的谱效关系,初步明确苍术主要燥性成分。方法采用GC-MS法对不同批次苍术(S1～S9)挥发油进行分析,以大鼠日均饮水量、全血黏度、肾脏中水通道蛋白2(AQP2)量为苍术燥性效应药效指标,采用灰色关联度分析法和正交偏最小二乘法相结合分析其谱效关系。结果苍术发挥燥性效应是多种成分共同作用的结果,对燥性作用药效贡献较大的3个峰分别为31、28、33号峰,经过谱库检索分析鉴定出3个峰对应的成分分别为β-桉叶醇、马兜铃烯、异愈创木醇。结论初步明确苍术主要燥性成分有β-桉叶醇、马兜铃烯、异愈创木醇,为苍术燥性物质基础研究提供参考。Objective To study the spectrum-effect relationship between GC-MS fingerprint of the volatile oil of Atractylodis Rhizoma and the dryness effect of Atractylodis Rhizoma, and clarify the main dryness components of Atractylodis Rhizoma. Methods The volatile oil of different batches of Atractylodis Rhizoma(S1-S9) were analyzed by GC-MS. Using the daily drinking amount of water, the viscosity of whole blood, and the content of aquaporin 2(AQP2) in the kidney of rats as the indexes of dryness effect ofAtractylodes Rhizome, the spectrum-effect relationship was analyzed by the combination of grey relational grade and orthogonal partial least square method. Results The dryness effect of Atractylodis Rhizoma was the combined action of multiple components, and the peaks of the major contribution to dryness effect were 31, 28, and 33, respectively. The corresponding components of the three peaks were β-eudesmol,(-)-aristolene, and bulnesol, which were identified by the spectral library retrieval analysis. Conclusion The main dryness effect components of Atractylodis Rhizoma are β-eudesmol,(-)-aristolene, and bulnesol, which provide a reference for investigating the material basis of dryness effect of Atractylodis Rhizoma.

关 键 词：苍术 灰色关联度 正交偏最小二乘法 燥性 谱效关系 Β-桉叶醇 马兜铃烯 异愈创木醇 

分 类 号：R285.5[医药卫生—中药学]