两株生防细菌对苦瓜枯萎病的防治效果及其定殖密度的研究  

Research on Control Effects of Two Biocontrol Agents Against Fusarium Wilt of Bitter Melon and the Root Colonization Ability

作　　者：刘东平[1] 张忠良 李嫚 文才艺[1] 朴凤植[2] 申顺善[1] LIU Dongping;ZHANG Zhongliang;LI Man;WEN Caiyi;PIAO Fengzhi;SHEN Shunshan(College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002;College of Horticulture, Henan Agricultural University,Zhengzhou, Henan 450002)

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]河南农业大学园艺学院,河南郑州450002

出　　处：《北方园艺》2019年第3期69-73,共5页Northern Horticulture

基　　金：国家重点研发计划资助项目(2017YFD0201100);公益性行业(农业)科研专项资助项目(201503110);国家农业产业技术体系建设专项资助项目(CARS-25-C-06)

摘　　要：以"如玉39"苦瓜为试材,利用皿内和盆栽试验,研究了生防细菌K18-5和KH48-5对苦瓜枯萎病菌的抑制活性和苦瓜枯萎病的防治效果,及其在苦瓜根系的定殖能力,以期为苦瓜枯萎病生物防治提供优质的生防资源。结果表明:K18-5和KH48-5均显著抑制枯萎病菌菌丝生长及其分生孢子的萌发;在盆栽试验中,K18-5和KH48-5对苦瓜枯萎病具有显著的防治效果,其防效分别达到75.27%和69.11%。K18-5和KH48-5均具有良好的根际定殖能力,在皿内采用双层滤纸法测定其定殖密度,分别为2.45×104 cfu·cm^(-1)和4.60×103 cfu·cm^(-1);在盆栽试验中,K18-5和KH48-5在苦瓜根部稳定保持1×103 cfu·g-1以上的定殖密度。‘Ruyu 39’bitter melon was used as material.In vitro and pot experiments were conducted to research on the inhibitory activities and control effect of two kinds of K18-5and KH48-5biocontrol agents against Fusarium oxysporumand their the root colonization abilities.In order to provide quality biological control resources for balsam pear wilt.The results showed that K18-5 and KH48-5 inhibited the mycelial growth and conidiospore germination of Fusarium oxysporum in vitro.In pot experiment,K18-5and KH48-5had significant control potentials against Fusarium wilt and the control efficiencies were 75.27% and 69.11% respectively.In addition,K18-5and KH48-5 had strong colonization abilities in rhizosphere of bitter melon,the colonization densities were 2.45×10^4 cfu·cm^-1 and 4.60×10^3 cfu·cm^-1 respectively with double layer filter paper method,in pot experiment,K18-5and KH48-5could sustain at high population densities in bitter melon root which were more than 1×103 cfu·g^-1.

关 键 词：生防细菌 苦瓜枯萎病 抑制 根际定殖 

分 类 号：S642.5[农业科学—蔬菜学]