日粮中添加不同水平的黄芪多糖对獭兔免疫力的影响  

Effect of astragalus polysaccharide on immunity of rex rabbit

作　　者：李楠[1] 肖建森 陈宝江[1] LI Nan;XIAO Jiansen;CHEN Baojiang(College of Animal Science and Technology of Hebei Agricultural University,Baoding 071000,China;Shandang Shenniu Animal Pharmaceutical Company,Dezhou 253000,China)

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]山东德州神牛药业公司,山东德州253000

出　　处：《黑龙江畜牧兽医》2019年第2期152-154,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家兔产业技术体系项目(CARS-44-B-3);河北省畜牧局项目"安全无抗畜禽饲料研究与开发"(2013-1-02);企业横向委托课题项目

摘　　要：为研究日粮中添加不同水平的黄芪多糖对獭兔免疫力的影响,试验选取50日龄、健康、体重相近的青年獭兔64只,随机分为4个组,每组16只,公母各半,对照组饲喂基础日粮,试验1~3组在饲喂基础日粮的基础上分别添加0. 10%、0. 15%、0. 20%的黄芪多糖,试验獭兔常规饲养管理及免疫,试验期28 d,第7,14,21,28采血清检测兔瘟抗体水平,第28天检测血液免疫细胞数、血清免疫球蛋白(Ig)含量。结果表明:试验1组血液免疫细胞数量除淋巴细胞数略高于对照组外,其他均低于对照组,但差异不显著(P>0. 05);试验2,3组免疫细胞数量除中性粒细胞外均高于对照组,但差异均不显著(P>0. 05)。试验1组除IgM含量与对照组相比稍有下降外,Ig G、Ig A含量均比对照组高,但差异不显著(P>0. 05);试验2,3组IgG、IgM、Ig A含量均高于对照组,且IgM、IgA含量与对照组相比差异显著(P<0. 05)。与对照组相比,免疫后试验1~3组兔瘟HI抗体效价上升速度快,高峰维持水平高,其中试验2,3组獭兔免疫两周后,效价均超过10. 0,达到高保护状态。说明日粮中添加黄芪多糖可以显著提高獭兔免疫水平,改善獭兔健康状态,适宜添加量为0. 15%~0. 20%。To study the effect of astragalus polysaccharides on health and immunity of rex rabbit,sixty four 50-days old rex rabbit were randomly divided into 4 groups and each group of 16 male half and female half. The control group feeding based diet while the diet of test 1,2,3 groups were added 0. 10%,0. 15%,0. 20% astragalus polysaccharides respectively. The routine management and immune program were used in the test. The test period was 28 days. The blood immune cell number,serum Ig content were detected on 28 th day,and rabbit antivirus level were measured 7 th,14 th,21 th,28 th day.The results showed that the blood immune cells number of test 1 group was less than the control group except for lymphocytes( P>0. 05). The number of immune cells in the test 2 and 3 groups were more than that in the control group except for neutrophil( P>0. 05). The Ig G levels and the Ig A levels in test 1 group were higher than those of the control group( P>0. 05) while the contents of Ig Min test 1 were lower than those in the control group. The contents of Ig G,Ig Mand Ig A in test 2 and 3 groups were higher than those in control group,and the contents of Ig M,Ig A were higher than those in control group significantly( P<0. 05). Compared with the control group,the antibody titer of RHDV of test groups increased rapidly and maintained a high peak level after immunization,and the antibody titer of test 2 and3 groups exceeded 10. 0 and reached a high protective state.It indicated that astragalus polysaccharide significantly improved humoral immunity and promoted the health status of rex rabbit. The appropriate additive amount of were 0. 15% ~ 0. 20%.

关 键 词：黄芪多糖 獭兔 免疫力 血液免疫细胞数 血清免疫球蛋白 兔瘟HI抗体 

分 类 号：S829.1[农业科学—畜牧学] S816.75[农业科学—畜牧兽医]